Development and validation of an analytical method for determination of pharmaceuticals in fish muscle based on QuEChERS extraction and SWATH acquisition using LC-QTOF-MS/MS system

This study aimed at developing an analytical method for the extraction and quantification of 21 pharmaceutical actives compounds (PhACs) present in fish muscle. Using Norwegian Atlantic salmon as matrix, two extraction methods for PhACs were tested: ultrasound extraction (USE) using methanol (MeOH),...

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Bibliographic Details
Published in:Talanta (Oxford) 2019-07, Vol.199, p.370-379
Main Authors: Peña-Herrera, J.M., Montemurro, N., Barceló, D., Pérez, S.
Format: Article
Language:English
Subjects:
Acetaminophen - analysis
Animals
Chromatography, High Pressure Liquid
Fish muscle
Food Contamination - analysis
Hybrid-QTOF
LC-HR-MS/MS
Muscles - chemistry
Pharmaceuticals
Propranolol - analysis
QuEChERS
Salmo salar
SWATH acquisition
Tandem Mass Spectrometry
Venlafaxine Hydrochloride - analysis
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Summary:This study aimed at developing an analytical method for the extraction and quantification of 21 pharmaceutical actives compounds (PhACs) present in fish muscle. Using Norwegian Atlantic salmon as matrix, two extraction methods for PhACs were tested: ultrasound extraction (USE) using methanol (MeOH), acetonitrile (MeCN) or a mixture of MeCN:MeOH (1:1, v/v) as extracting solvents, and QuEChERS method using three different extraction salts. After selecting QuEChERS Original as extracting method of the analytes, three different clean-up methods were evaluated with respect to their efficiency to remove coextracted fat and lipids such as Enhanced Matrix Removal (EMR) and HLB prime. The dispersive-SPE EMR yielded the best recoveries for 21 of 27 analytes. PhACs were quantified by UPLC-MS/MS using SWATH acquisition mode. The method was validated in terms of recoveries, accuracy, linearity, precision, matrix effects at three levels of concentration: 25, 200 and 500 ng g−1 dw of fish muscle. For the majority of the analytes the recoveries were over 70%. Finally, the validated method was applied to natural riverine fish from the Evrotas river (Greece) and the Adige river (Italy) with positive findings for acetaminophen, propranolol, and venlafaxine reaching concentrations as high as 80 ng g−1 of muscle. [Display omitted] •Development of a rapid method for the determination of 21 PhACs in fish muscle.•Two extraction methods, 3 extracting salts, and 3 clean-up of extracts were compared.•The method is based on QuEChERS extraction and clean-up, followed by UPLC–HRMS.•The validated method was applied to riverine European fish.
ISSN:0039-9140
1873-3573
DOI:10.1016/j.talanta.2019.01.119