Role of GM-CSF in a mouse model of experimental autoimmune prostatitis

The etiology of chronic prostatitis/chronic pelvic pain syndrome (CP/CPPS) is still unknown. Granulocyte macrophage colony-stimulating factor (GM-CSF) has been shown to play an important role in the development of autoimmune and inflammatory diseases. Here, we investigated the expression and functio...

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Bibliographic Details
Published in:American journal of physiology. Renal physiology 2019-07, Vol.317 (7), p.F23-F29
Main Authors: Liu, Yaxiao, Li, Yan, Liu, Qinggang, Wu, Zonglong, Cui, Jianfeng, Zhu, Kejia, Zhao, Hongda, Zhou, Changkuo, Shi, Benkang
Format: Article
Language:English
Subjects:
Animals
Autoimmune diseases
Autoimmune Diseases - etiology
Autoimmune Diseases - physiopathology
Cerebrospinal fluid
Chemokines
Chronic Pain
Colony-stimulating factor
Disease Models, Animal
Dorsal root ganglia
Etiology
Ganglia, Spinal - chemistry
Ganglia, Spinal - metabolism
Gene Expression
Granulocyte-macrophage colony-stimulating factor
Granulocyte-Macrophage Colony-Stimulating Factor - deficiency
Granulocyte-Macrophage Colony-Stimulating Factor - genetics
Granulocyte-Macrophage Colony-Stimulating Factor - physiology
Humans
Immunohistochemistry
Inflammatory diseases
Ligands
Male
Mice
Mice, Knockout
Nerve growth factor
Pain
Pain perception
Pelvic Pain
Prostate
Prostate - chemistry
Prostate - metabolism
Prostatitis
Prostatitis - immunology
Prostatitis - physiopathology
Receptors, Granulocyte-Macrophage Colony-Stimulating Factor - analysis
Receptors, Granulocyte-Macrophage Colony-Stimulating Factor - genetics
RNA, Messenger - analysis
Secretions
Semen - chemistry
Signal transduction
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Summary:The etiology of chronic prostatitis/chronic pelvic pain syndrome (CP/CPPS) is still unknown. Granulocyte macrophage colony-stimulating factor (GM-CSF) has been shown to play an important role in the development of autoimmune and inflammatory diseases. Here, we investigated the expression and function of GM-CSF in patients with CP/CPPS and in a mouse model of experimental autoimmune prostatitis (EAP). GM-CSF mRNA levels were detected in expressed prostatic secretions samples from patients with CP/CPPS and in prostate tissue from a mouse model of EAP. The expression of GM-CSF receptor in mouse prostate and dorsal root ganglia were determined using PCR and immunohistochemistry. Behavioral testing and inflammation scoring were performed to evaluate the role of GM-CSF in disease development and symptom severity of EAP using GM-CSF knockout mice. mRNA levels of putative nociceptive and inflammatory markers were measured in the prostate after the induction of EAP. Elevated GM-CSF mRNA levels were observed in expressed prostatic secretions samples from patients with CP/CPPS compared with healthy volunteers. GM-CSF mRNA was also significantly increased in prostate tissue of the EAP mice model. The expression of GM-CSF receptors was confirmed in mouse prostate and dorsal root ganglia. GM-CSF knockout mice showed fewer Infiltrating leukocytes and pain symptoms after the induction of EAP. Deletion of GM-CSF significantly diminished EAP-induced increases of chemokine (C-C motif) ligand 2, chemokine (C-C motif) ligand 3, and nerve growth factor mRNA expression. The results indicated that GM-CSF plays a functional role in the pathogenesis of EAP. GM-CSF may function as a signaling mediator for both inflammation and pain transduction in CP/CPPS.
ISSN:1931-857X
1522-1466
DOI:10.1152/ajprenal.00013.2018