Quantitation of Glutathione by Quinoline-5, 8-Dione-Based Tag Strategy Using MALDI Mass Spectrometry

In the present work, we developed an UV-absorptive and highly reactive tag aromatic molecule, quinoline-5,8-dione (QLD), for robust quantitative analysis of GSH by matrix-assisted laser desorption/ionization (MALDI) mass spectrometry. The QLD could react with GSH with high efficiency at room tempera...

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Bibliographic Details
Published in:Journal of the American Society for Mass Spectrometry 2019-04, Vol.30 (4), p.625-633
Main Authors: Guo, Liming, Xiao, Chunsheng, Wang, Sheng, Gao, Tianyang, Ling, Ling, Guo, Xinhua
Format: Article
Language:English
Subjects:
Absorptivity
Alanine
Analytical Chemistry
Bioinformatics
Biotechnology
Chemistry
Chemistry and Materials Science
Colorimetry
Diketones
Glutamic acid
Glutathione
Ionization
Ions
Linearity
Mass spectrometry
Organic Chemistry
Proteomics
Quantitative analysis
Quinoline
Research Article
Room temperature
Scientific imaging
Spectroscopy
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Summary:In the present work, we developed an UV-absorptive and highly reactive tag aromatic molecule, quinoline-5,8-dione (QLD), for robust quantitative analysis of GSH by matrix-assisted laser desorption/ionization (MALDI) mass spectrometry. The QLD could react with GSH with high efficiency at room temperature, and the resultant QLD-GSH conjugate could be readily detected by MALDI MS without interferences. By using the QLD tag, the detection limit of GSH was lowered to 10 fmol μL −1 , which was four orders of magnitude higher than that detected without using the QLD tag. Furthermore, accurate quantitative measurements of GSH in solution were successfully demonstrated by using glutamic acid-cysteine-alanine (ECA) as an internal standard. By properly adjusting the ECA concentrations, the intensity ratio value of QLD-tagged GSH (QLD-GSH) to QLD-tagged ECA (QLD-ECA) displayed a good linearity with GSH concentrations in a broad range from 4 to 4000 μM. Finally, the GSH level in HeLa cell lysates was also successfully detected, and the results are consistent with that obtained by a colorimetric assay. In summary, the proposed QLD-based tag method should be a rapid, cost-/time-effective, and sensitive new method for quantitative determination of GSH by MALDI MS. Graphical Abstract
ISSN:1044-0305
1879-1123
DOI:10.1007/s13361-019-02135-4