Formononetin Antagonizes the Interleukin-1β-Induced Catabolic Effects Through Suppressing Inflammation in Primary Rat Chondrocytes

In the present study, we demonstrated the anti-catabolic effects of formononetin, a phytoestrogen derived from herbal plants, against interleukin-1β (IL-1β)-induced severe catabolic effects in primary rat chondrocytes and articular cartilage. Formononetin did not affect the viability of primary rat...

Full description

Saved in:
Bibliographic Details
Published in:Inflammation 2019-08, Vol.42 (4), p.1426-1440
Main Authors: Cho, In-A, Kim, Tae-Hyeon, Lim, HyangI, Park, Jong-Hyun, Kang, Kyeong-Rok, Lee, Sook-Young, Kim, Chun Sung, Kim, Do Kyung, Kim, Heung-Joong, Yu, Sun-Kyoung, Kim, Su-Gwan, Kim, Jae-Sung
Format: Article
Language:English
Subjects:
Animals
Biomedical and Life Sciences
Biomedicine
Cartilage (articular)
Cartilage diseases
Cartilage, Articular - drug effects
Cells, Cultured
Chemokines
Chondrocytes
Chondrocytes - pathology
Cyclooxygenase-2
Cytokines
Drug Antagonism
Fractalkine
IL-1β
Immunology
Inflammation
Inflammation - drug therapy
Interleukin 6
Interleukin-1beta - antagonists & inhibitors
Interleukin-1beta - pharmacology
Internal Medicine
Interstitial collagenase
Isoflavones - antagonists & inhibitors
Isoflavones - pharmacology
Macrophage inflammatory protein
Matrix metalloproteinase
Metabolism - drug effects
Metalloproteinase
Monocyte chemoattractant protein
Monocyte chemoattractant protein 1
Monocytes
Nitric oxide
Nitric-oxide synthase
Original Article
Osteoarthritis
Osteoarthritis - prevention & control
Oxidative stress
Pathology
Pharmacology/Toxicology
Phytoestrogens - pharmacology
Prostaglandin E2
Rats
Rheumatology
Stromelysin 1
Tumor necrosis factor-α
Vascular endothelial growth factor
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:In the present study, we demonstrated the anti-catabolic effects of formononetin, a phytoestrogen derived from herbal plants, against interleukin-1β (IL-1β)-induced severe catabolic effects in primary rat chondrocytes and articular cartilage. Formononetin did not affect the viability of primary rat chondrocytes in both short- (24 h) and long-term (21 days) treatment periods. Furthermore, formononetin effectively antagonized the IL-1β-induced catabolic effects including the decrease in proteoglycan content, suppression of pericellular matrix formation, and loss of proteoglycan through the decreased expression of cartilage-degrading enzymes like matrix metalloproteinase (MMP)-13, MMP-1, and MMP-3 in primary rat chondrocytes. Moreover, catabolic oxidative stress mediators like nitric oxide, inducible nitric oxide synthase, cyclooxygenase-2, and prostaglandin E 2 were significantly downregulated by formononetin in primary rat chondrocytes treated with IL-1β. Sequentially, the upregulation of pro-inflammatory cytokines (like IL-1α, IL-1β, IL-6, and tumor necrosis factor α), chemokines (like fractalkine, monocyte chemoattractant protein-1, and macrophage inflammatory protein-3α), and vascular endothelial growth factor were significantly downregulated by formononetin in primary rat chondrocytes treated with IL-1β. These data suggest that formononetin may suppress IL-1β-induced severe catabolic effects and osteoarthritic condition. Furthermore, formononetin may be a promising candidate for the treatment and prevention of osteoarthritis.
ISSN:0360-3997
1573-2576
DOI:10.1007/s10753-019-01005-1