Phylogenomic characterization of red seabream iridovirus from Florida pompano Trachinotus carolinus maricultured in the Caribbean Sea

Between 2010 and 2016, six mortality events were observed in Florida pompano ( Trachinotus carolinus ) maricultured in the Dominican Republic. Histopathological examination and conventional PCR confirmed a megalocytivirus (MCV) infection in each case. Subsequently, next-generation sequencing and phy...

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Bibliographic Details
Published in:Archives of virology 2019-04, Vol.164 (4), p.1209-1212
Main Authors: Koda, Samantha A., Subramaniam, Kuttichantran, Pouder, Deborah B., Yanong, Roy P., Waltzek, Thomas B.
Format: Article
Language:English
Subjects:
Annotated Sequence Record
Biomedical and Life Sciences
Biomedicine
DNA sequencing
Fish
Genomes
Infectious Diseases
Medical Microbiology
Next-generation sequencing
Nucleotide sequence
Open reading frames
Trachinotus carolinus
Virology
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Summary:Between 2010 and 2016, six mortality events were observed in Florida pompano ( Trachinotus carolinus ) maricultured in the Dominican Republic. Histopathological examination and conventional PCR confirmed a megalocytivirus (MCV) infection in each case. Subsequently, next-generation sequencing and phylogenomic analyses confirmed that MCV DNA was present in the infected pompano tissue samples from 2010, 2014, and 2016, and each was determined to be red seabream iridovirus (RSIV). Annotation of the RSIV genome sequences identified 121 open reading frames, and BLASTN analysis revealed the highest nucleotide sequence identity (> 99%) to a RSIV clade 1 MCV isolated from a moribund red seabream ( Pagrus major ) maricultured in Japan. These cases represent the first fully sequenced RSIV genomes detected outside of Asia and are the earliest reports of MCV infections in Florida pompano. This recent geographical expansion of RSIV warrants further attention to determine its potential economic and ecological impact.
ISSN:0304-8608
1432-8798
DOI:10.1007/s00705-019-04155-7