Hollow silica microspheres as robust immobilization carriers

[Display omitted] •MATSPHERES® can substitute a golden standard SBA for enzyme immobilization.•Immobilisation of enzymes on MATSPHERES® enables their employment in flow regime.•Immobilised formulation of lipase are efficient catalysts for sugar ester synthesis.•Immobilised EbDH performs better on OH...

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Bibliographic Details
Published in:Bioorganic chemistry 2019-12, Vol.93, p.102813-102813, Article 102813
Main Authors: Snoch, Wojciech, Tataruch, Mateusz, Zastawny, Olga, Cichoń, Ewelina, Gosselin, Mathilde, Cabana, Hubert, Guzik, Maciej
Format: Article
Language:English
Subjects:
Batch reactor
Biocatalysis
Ethylbenzene dehydrogenase
Flow reactor
Hollow silica microspheres
Immobilization
Lipase
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Summary:[Display omitted] •MATSPHERES® can substitute a golden standard SBA for enzyme immobilization.•Immobilisation of enzymes on MATSPHERES® enables their employment in flow regime.•Immobilised formulation of lipase are efficient catalysts for sugar ester synthesis.•Immobilised EbDH performs better on OH-type supports in prolonged anaerobic operation. Hollow silica microspheres provide an ideal solid support for enzyme immobilization. We tested one of the newest development, namely MATSPHERES®, a silica openwork material as a carrier for the covalent immobilization of enzymes used to synthesize bioactive compounds. Two model enzymes – ethylbenzene dehydrogenase and EL070 lipase – were considered. They belong to two different enzyme classes and catalyse reactions taking place in various environments (aqueous and non-aqueous, aerobic and anaerobic). The enzymes were immobilized by covalent bonds (via divinyl sulfone and glutaraldehyde) on new silica material. Effectiveness of immobilization processes on the spheres grafted with amine groups and on the analogues without functionalization was determined for both enzymes. Microspheres were characterized morphologically and also their mechanical stability was examined during exposure to varying physical conditions. It was shown that MATSPHERES® due to their openwork structure and relative stability under batch and flow conditions can be a competitive SBA support for enzyme immobilization and production of bioactive compounds.
ISSN:0045-2068
1090-2120
DOI:10.1016/j.bioorg.2019.02.038