Recovery of an Acanthamoeba strain with two group I introns in the nuclear 18S rRNA gene

Nuclear group I introns are parasitic mobile genetic elements occurring in the ribosomal RNA genes of a large variety of microbial eukaryotes. In Acanthamoeba, group I introns were found occurring in the 18S rDNA at four distinct insertion sites. Introns are present as single elements in various str...

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Bibliographic Details
Published in:European journal of protistology 2019-04, Vol.68, p.88-98
Main Authors: Corsaro, Daniele, Köhsler, Martina, Venditti, Danielle, Rott, Marilise B., Walochnik, Julia
Format: Article
Language:English
Subjects:
Acanthamoeba
Acanthamoeba lenticulata
Group I intron
Nuclear IC1 introns
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Summary:Nuclear group I introns are parasitic mobile genetic elements occurring in the ribosomal RNA genes of a large variety of microbial eukaryotes. In Acanthamoeba, group I introns were found occurring in the 18S rDNA at four distinct insertion sites. Introns are present as single elements in various strains belonging to four genotypes, T3 (A. griffini), T4 (A. castellanii complex), T5 (A. lenticulata) and T15 (A. jacobsi). While multiple introns can frequently be found in the rDNA of several algae, fungi and slime moulds, they are usually rare and present as single elements in amoebae. We reported herein the characterization of an A. lenticulata strain containing two introns in its 18S rDNA. They are located to already known sites and show basal relationships with respective homologous introns present in the other T5 strains. This is the first and unique reported case of multiple nuclear introns in Acanthamoeba.
ISSN:0932-4739
1618-0429
DOI:10.1016/j.ejop.2019.01.007