QTL analysis of durable stripe rust resistance in the North American winter wheat cultivar Skiles

Key message This study determined the effects of growth stage and temperature on expression of high-temperature adult-plant resistance to stripe rust, mapped six QTL for durable resistance in winter wheat Skiles using a doubled haploid population, and selected breeding lines with different combinati...

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Bibliographic Details
Published in:Theoretical and applied genetics 2019-06, Vol.132 (6), p.1677-1691
Main Authors: Liu, L., Yuan, C. Y., Wang, M. N., See, D. R., Zemetra, R. S., Chen, X. M.
Format: Article
Language:English
Subjects:
Agriculture
Basidiomycota - physiology
Biochemistry
Biomedical and Life Sciences
Biotechnology
Breeding
Chromosome Mapping
Chromosomes, Plant - genetics
Control
Cultivars
Disease Resistance - genetics
Diseases and pests
Gene mapping
Genetic aspects
Genotype
Genotyping
Growth stage
High temperature
Life Sciences
Low temperature
Marker-assisted selection
Methods
Original Article
Phenotypic variations
Plant Biochemistry
Plant Breeding/Biotechnology
Plant Diseases - genetics
Plant Diseases - microbiology
Plant Genetics and Genomics
Plant immunology
Plant resistance
Polymorphism, Single Nucleotide
Population
Quantitative Trait Loci
Rust diseases
Seasons
Single-nucleotide polymorphism
Stripe rust
Temperature
Temperature effects
Triticum - genetics
Triticum - microbiology
Triticum aestivum
United States
Wheat
Winter wheat
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Summary:Key message This study determined the effects of growth stage and temperature on expression of high-temperature adult-plant resistance to stripe rust, mapped six QTL for durable resistance in winter wheat Skiles using a doubled haploid population, and selected breeding lines with different combinations of the QTL using marker-assisted selection. The winter wheat cultivar Skiles has a high level of high-temperature adult-plant (HTAP) resistance to stripe rust caused by Puccinia striiformis f. sp. tritici ( Pst ). The Skiles HTAP resistance was highly effective at the adult-plant stage even under low temperatures, but high temperatures induced earlier expression and increased levels of resistance. To map resistance genes, Skiles was crossed with the susceptible cultivar Avocet S and a doubled haploid (DH) population was developed. The DH population was tested in fields at Pullman, WA, in 2016, 2017 and 2018, Mount Vernon, WA, in 2017 and 2018 under natural infection, and an environmentally controlled greenhouse at the adult-plant stage with the currently predominant race PSTv-37. The population was genotyped using the 90 K Illumina iSelect wheat SNP chip and selected SSR markers on specific chromosomes. In total, 2526 polymorphic markers were used for QTL mapping and six QTL were detected. Two of the six QTL had major effects across all environments, with one mapped on chromosome 3BS, explaining up to 28.2% of the phenotypic variation and the other on chromosome 4BL, explaining up to 41.8%. Minor QTL were mapped on chromosomes 1BL, 5AL, 6B and 7DL. Genotyping 140 wheat cultivars from the US Pacific Northwest revealed high polymorphism of markers for five of the QTL, and five highly resistant lines with the five QTL were selected from Skiles-derived breeding lines using the markers. This study demonstrated that multiple QTL with mostly additive effects contributed to the high-level HTAP resistance in Skiles.
ISSN:0040-5752
1432-2242
DOI:10.1007/s00122-019-03307-2