“Solvent/non-solvent” treatment as a method for non-covalent immobilization of gelatin on the surface of poly(l-lactic acid) electrospun scaffolds

[Display omitted] •PLLA fibers were non-covalently coated with gelatin via toluene/ethanol treatment.•Gelatin immobilization enhanced PLLA hydrophilicity, strength and biocompatibility.•Thickness of gelatin-containing coating was found at ≈400 nm.•Optimal time of gelatin immobilization was found at...

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Published in:Colloids and surfaces, B, Biointerfaces B, Biointerfaces, 2019-05, Vol.177, p.137-140
Main Authors: Goreninskii, S.I., Guliaev, R.O., Stankevich, K.S., Danilenko, N.V., Bolbasov, E.N., Golovkin, A.S., Mishanin, A.I., Filimonov, V.D., Tverdokhlebov, S.I.
Format: Article
Language:English
Subjects:
Biocompatible Materials - chemistry
Biocompatible Materials - pharmacology
Cell Adhesion - drug effects
Cells, Cultured
Electrospun scaffolds
Gelatin
Gelatin - chemistry
Gelatin - pharmacology
Humans
Microscopy, Fluorescence
Particle Size
Polyesters - chemistry
Polyesters - pharmacology
Polylactic acid
Solvents - chemistry
Stem Cells - drug effects
Surface modification
Surface Properties
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Summary:[Display omitted] •PLLA fibers were non-covalently coated with gelatin via toluene/ethanol treatment.•Gelatin immobilization enhanced PLLA hydrophilicity, strength and biocompatibility.•Thickness of gelatin-containing coating was found at ≈400 nm.•Optimal time of gelatin immobilization was found at 5 min.•Suggested method doesn't require special equipment or high-cost reagents. In the present study, we report a simple and efficient method of gelatin immobilization on the surface of PLA electrospun fibers using pre-treatment with a mixture of toluene and ethanol allowing to form swelled surface layer followed by gelatin adsorption from its solution in PBS. Our results demonstrate that gelatin immobilization leads to a decrease in the water contact angle from 120° to 0°, enhances scaffold strength up to 50%, and doubles the number of adhered cells and their average area. We observed that the maximum amount of gelatin (0.07 ± 0.01 mg per cm3 of the scaffold) was immobilized during the first five minutes of exposure to the gelatin solution. Modified scaffolds demonstrated increased strength.
ISSN:0927-7765
1873-4367
DOI:10.1016/j.colsurfb.2019.01.060