Vertical transmission of Neospora caninum in bovine fetuses from a slaughterhouse in Brazil

Neospora caninum is considered as one of the main causes of reproductive failure in cattle. Vertical transmission is the main route of infection in the bovine host and plays an important role in maintaining the parasite in the herd. Molecular detection of N. caninum is important to determine the occ...

Full description

Saved in:
Bibliographic Details
Published in:Tropical animal health and production 2019-07, Vol.51 (6), p.1751-1755
Main Authors: de Aquino Diniz, Lanna Vivien, Minutti, Ana Flávia, de Souza Lima Nino, Beatriz, Costa, Letícia Ramos, Bosculo, Maria Rachel Melo, de Almeida, Breno Fernando Martins, Garcia, João Luis, de Barros, Luiz Daniel
Format: Article
Language:English
Subjects:
Abattoirs
Animals
Antibodies
Antibodies, Protozoan - blood
Biological Assay
Biomedical and Life Sciences
Bovidae
Brain
Brazil - epidemiology
Cattle
Cattle Diseases - epidemiology
Cattle Diseases - parasitology
Cattle Diseases - transmission
Coccidiosis - epidemiology
Coccidiosis - transmission
Coccidiosis - veterinary
Diagnostic systems
Female
Fetus - parasitology
Fetuses
Fluorescence
Fluorescent Antibody Technique, Indirect - veterinary
Fluorescent antibody test
Genetic diversity
Heart
Indirect fluorescent antibody test
Infectious Disease Transmission, Vertical - veterinary
Life Sciences
Neospora - immunology
Neospora - isolation & purification
Neospora caninum
Parasites
Polymerase Chain Reaction
Pregnancy
Protozoa
Reproductive failure
Short Communications
Tissues
Veterinary Medicine/Veterinary Science
Zoology
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Neospora caninum is considered as one of the main causes of reproductive failure in cattle. Vertical transmission is the main route of infection in the bovine host and plays an important role in maintaining the parasite in the herd. Molecular detection of N. caninum is important to determine the occurrence of the disease and to evaluate the genetic diversity of the parasite. The present study aimed at assessing the vertical transmission of N. caninum using molecular techniques to detect the parasite in tissue samples from bovine fetuses collected in a slaughterhouse in the state of São Paulo, Brazil. Seventy fetuses and 70 blood samples from pregnant cows were collected in a slaughtering line. Fresh samples of heart and brain tissue from fetuses were analyzed using molecular assays. Serum samples from fetuses and cows were subjected to an indirect fluorescent antibody test (IFAT) to detect antibodies against N. caninum . Nested PCR targeting the internal transcriber 1 (ITS1) region of the protozoan organism was used in the molecular testing. From the total of fetuses examined, 71.42% were positive for N. caninum by PCR. A higher number of heart samples (47.1%) were positive for the parasite using this technique. Antibodies against the protozoa were detected in 12.9% of serum samples of cows; 2.8% of fetuses were seropositive for this pathogen. Our results show that vertical transmission of N. caninum occurs in cattle from this region of Brazil, and that the use of different diagnostic techniques contributes to successful diagnosis of congenital transmission of the parasite in cattle.
ISSN:0049-4747
1573-7438
DOI:10.1007/s11250-019-01828-y