A novel monoclonal antibody against enolase antigen of Aspergillus fumigatus protects experimental aspergillosis in mice

Aspergillus fumigatus is one of the most common opportunistic fungal pathogen responsible for a variety of diseases in human from allergic bronchopulmonary aspergillosis to chronic pulmonary aspergillosis mostly in immunocompromised patients.In this study, one monoclonal antibody MAb R-5 (IgM) raise...

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Bibliographic Details
Published in:FEMS microbiology letters 2019-02, Vol.366 (3), p.1
Main Authors: Yadav, Ravindra Kumar, Shukla, P K
Format: Article
Language:English
Subjects:
Allergic bronchopulmonary aspergillosis
Analysis
Antigens
Aspergillosis
Aspergillus
Aspergillus fumigatus
Care and treatment
Cell surface
Fungal infections
Fungicidal activity
Fungicides
Germination
Hyphae
Identification and classification
Immunofluorescence
Immunoglobulin M
Kidneys
Microbiology
Monoclonal antibodies
Opportunist infection
Phosphopyruvate hydratase
Properties
Spore germination
Spores
VOCs
Volatile organic compounds
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Summary:Aspergillus fumigatus is one of the most common opportunistic fungal pathogen responsible for a variety of diseases in human from allergic bronchopulmonary aspergillosis to chronic pulmonary aspergillosis mostly in immunocompromised patients.In this study, one monoclonal antibody MAb R-5 (IgM) raised against enolase cell surface protein of A. fumigatus exhibited significant inhibition of spore germination in A. fumigatus (88.3%), A. flavus (57.4%), and A. niger (30.6%). The MAb R-5 also showed in vitro fungicidal activity against these species as follows; A. fumigatus (24.1%), A. flavus (13.3%), and A. niger (8.8%). These findings were supported by the indirect immunofluorescence microscopy where the antibody showed binding with germinated spores and hyphae of A. fumigatus as well as A. flavus and A. niger.In vivo protective effect of MAb R-5 was evaluated in BALB/c mice challenged intravenously with A. fumigatus spores where a significant reduction in CFU (85.9%) was observed in kidney tissue. The mean survival time of mice treated with MAb R-5 (18.5days) was also enhanced compared to control (6.5days). These results indicate that MAb R-5 could be valuable in diagnosis as well as in the treatment of broad range of Aspergillus infections.
ISSN:1574-6968
0378-1097
1574-6968
DOI:10.1093/femsle/fnz015