Synergistic effect of hyperbaric oxygen therapy with PTH [1–34] on calvarial bone graft in irradiated rat

Objective To determine the synergistic effect of parathyroid hormone (PTH) [1–34] in combination with hyperbaric oxygen (HBO) on bone graft in a rat calvarial bone defect model under impaired osteogenic conditions. Materials and Methods Twenty‐four rats were divided into three groups. Localized radi...

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Bibliographic Details
Published in:Oral diseases 2019-04, Vol.25 (3), p.822-830
Main Authors: Park, Kyeong‐Mee, Hu, Kyung‐Seok, Choi, Hyunmin, Oh, Seo‐Eun, Kim, Hyeong‐Il, Park, Wonse, Kim, Sungtae
Format: Article
Language:English
Subjects:
animal model
Animals
bone graft
Bone grafts
Bone healing
bone regeneration
Bone Substitutes
Calcium phosphates
Calvaria
calvarial defect
Combined Modality Therapy
Hydroxyapatites
Hyperbaric oxygen therapy
Hyperbaric Oxygenation
Male
Osteogenesis - drug effects
Osteogenesis - radiation effects
Parathyroid
Parathyroid hormone
Peptide Fragments - therapeutic use
Phosphates
Rats
Rats, Sprague-Dawley
Skin & tissue grafts
Skull - diagnostic imaging
Skull - pathology
Skull - physiology
Skull - surgery
Teriparatide - analogs & derivatives
Teriparatide - therapeutic use
X-Ray Microtomography
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Summary:Objective To determine the synergistic effect of parathyroid hormone (PTH) [1–34] in combination with hyperbaric oxygen (HBO) on bone graft in a rat calvarial bone defect model under impaired osteogenic conditions. Materials and Methods Twenty‐four rats were divided into three groups. Localized radiation with a single 12 Gy dose was administered to the calvaria. Four weeks after radiation, calvarial circular defects were created in the parietal bones. All defects were filled with biphasic calcium phosphate. After the bone graft, PTH [1–34] was injected subcutaneously, and HBO was administered. At 6 weeks after the bone graft, the rats were sacrificed, and specimens were harvested. Results Histomorphometric evaluation showed that the percentage of new bone area was higher in the PTH and PTH/HBO groups than in the control group. The percent residual material area was decreased in the PTH/HBO group compared with the control group. The percentage blood vessel number was highest in the PTH group. Micro‐CT evaluation showed that the new bone volume was highest in the PTH/HBO group. The residual material volume was lowest in the PTH/HBO group. Conclusion Within the limitations of this study, our data indicate that PTH combined with HBO may reverse radiation‐induced impairment of bone healing.
ISSN:1354-523X
1601-0825
DOI:10.1111/odi.13037