Polyhydroxybutyrate production in halophilic marine bacteria Vibrio proteolyticus isolated from the Korean peninsula

Polyhydroxybutyrates (PHB) are biodegradable polymers that are produced by various microbes, including Ralstonia, Pseudomonas , and Bacillus species. In this study, a Vibrio proteolyticus strain, which produces a high level of polyhydroxyalkanoate (PHA), was isolated from the Korean marine environme...

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Bibliographic Details
Published in:Bioprocess and biosystems engineering 2019-04, Vol.42 (4), p.603-610
Main Authors: Hong, Ju-Won, Song, Hun-Suk, Moon, Yu-Mi, Hong, Yoon-Gi, Bhatia, Shashi Kant, Jung, Hye-Rim, Choi, Tae-Rim, Yang, Soo-yeon, Park, Hyung-Yeon, Choi, Yong-Keun, Yang, Yung-Hun
Format: Article
Language:English
Subjects:
Bacteria
Biodegradability
Biodegradation
Biotechnology
Carbon
Carbon sources
Chemistry
Chemistry and Materials Science
Chromatography
Engineering
Environmental Engineering/Biotechnology
Enzymes
Food Science
Fructose
Industrial and Production Engineering
Industrial Chemistry/Chemical Engineering
Marine environment
Nitrogen
Nitrogen sources
Polyhydroxyalkanoates
Polyhydroxyalkanoic acid
Polyhydroxybutyrate
Polyhydroxybutyric acid
Polymers
Propionic acid
Research Paper
Salinity
Sodium
Sodium chloride
Substrates
Vibrio
Waterborne diseases
Yeast
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Summary:Polyhydroxybutyrates (PHB) are biodegradable polymers that are produced by various microbes, including Ralstonia, Pseudomonas , and Bacillus species. In this study, a Vibrio proteolyticus strain, which produces a high level of polyhydroxyalkanoate (PHA), was isolated from the Korean marine environment. To determine optimal growth and production conditions, environments with different salinity, carbon sources, and nitrogen sources were evaluated. We found that the use of a medium containing 2% (w/v) fructose, 0.3% (w/v) yeast extract, and 5% (w/v) sodium chloride (NaCl) in M9 minimal medium resulted in high PHA content (54.7%) and biomass (4.94 g/L) over 48 h. Addition of propionate resulted in the production of poly(3-hydroxybutyrate- co -3-hydroxyvalerate) (P(HB-co-HV)) copolymer as propionate acts as a precursor for the HV unit. In these conditions, the bacteria produced poly(3-hydroxybutyrate- co -3-hydroxyvalerate) containing a 15.8% 3HV fraction with 0.3% propionate added as the substrate. To examine the possibility of using unsterilized media with high NaCl content for PHB production, V. proteolyticus was cultured in sterilized and unsterilized conditions. Our results indicated a higher growth, leading to a dominant population in unsterilized conditions and higher PHB production. This study showed the conditions for halophilic PHA producers to be later implemented at a larger scale.
ISSN:1615-7591
1615-7605
DOI:10.1007/s00449-018-02066-6