Three-dimensional tumor visualization of invasive breast carcinomas using whole-mount serial section histopathology: implications for tumor size assessment

Purpose Linear tumor size (T-size) estimated with conventional histology informs breast cancer management. Previously we demonstrated significant differences in margin and focality estimates using conventional histology versus digital whole-mount serial sections (WMSS). Using WMSS we can measure T-s...

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Bibliographic Details
Published in:Breast cancer research and treatment 2019-04, Vol.174 (3), p.669-677
Main Authors: Clarke, G. M., Holloway, C. M. B., Zubovits, J. T., Nofech-Mozes, S., Murray, M., Liu, K., Wang, D., Kiss, A., Yaffe, M. J.
Format: Article
Language:English
Subjects:
Breast cancer
Breast carcinoma
Breast Neoplasms - diagnostic imaging
Breast Neoplasms - pathology
Breast Neoplasms - surgery
Cancer research
Cancer therapies
Carcinoma
Comparative analysis
Female
Health sciences
Histochemistry
Histological Techniques - methods
Histology
Histopathology
Humans
Imaging, Three-Dimensional - methods
Lumpectomy
Lymphatic system
Margins of Excision
Mastectomy, Segmental
Medical prognosis
Medical research
Medicine
Medicine & Public Health
Metastasis
Neoplasm Invasiveness - diagnostic imaging
Neoplasm Invasiveness - pathology
Oncology
Pathology
Preclinical Study
Research centers
Specimen Handling
Studies
Surgery
Tumor Burden
Tumors
Visualization (Computer)
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Summary:Purpose Linear tumor size (T-size) estimated with conventional histology informs breast cancer management. Previously we demonstrated significant differences in margin and focality estimates using conventional histology versus digital whole-mount serial sections (WMSS). Using WMSS we can measure T-size or volume. Here, we compare WMSS T-size with volume, and with T-size measured conventionally. We also compare the ellipsoid model for calculating tumor volume to direct, WMSS measurement. Methods Two pathologists contoured regions of invasive carcinoma and measured T-size from both WMSS and (simulated) conventional sections in 55 consecutive lumpectomy specimens. Volume was measured directly from the contours. Measurements were compared using the paired t -test or Spearman’s rank-order correlation. A five-point ‘border index’ was devised and assigned to each case to parametrize tumor shape considering ‘compactness’ or cellularity. Tumor volumes calculated assuming ellipsoid geometry were compared with direct, WMSS measurements. Results WMSS reported significantly larger T-size than conventional histology in the majority of cases [61.8%, 34/55; means = (2.34 cm; 1.99 cm), p  
ISSN:0167-6806
1573-7217
DOI:10.1007/s10549-018-05122-7