Insulin‐like growth factor‐II mRNA‐binding protein 3 immunocytochemical expression in direct endometrial brushings: Possible diagnostic help in endometrial cytology

Introduction This study evaluated the immunocytochemical (ICC) expression of IMP3 in direct endometrial brushings processed as liquid‐based cytology (LBC) samples of endometrioid adenocarcinoma (EAC), serous carcinoma (ESC) and surface papillary syncytial change (SPSC) with endometrial glandular and...

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Published in:Cytopathology (Oxford) 2019-03, Vol.30 (2), p.215-222
Main Authors: Norimatsu, Yoshiaki, Yanoh, Kenji, Maeda, Yoshinobu, Irino, Satoshi, Hirai, Yasuo, Fulciniti, Franco, Kobayashi, Tadao K.
Format: Article
Language:English
Subjects:
Adenocarcinoma
Adenocarcinoma - diagnosis
Adenocarcinoma - genetics
Adenocarcinoma - pathology
Aged
Benign
Biomarkers, Tumor - genetics
Cellular biology
Cystadenocarcinoma, Serous - diagnosis
Cystadenocarcinoma, Serous - genetics
Cystadenocarcinoma, Serous - pathology
Cytodiagnosis - methods
Cytology
Diagnosis, Differential
endometrial cytology
Endometrial Neoplasms - diagnosis
Endometrial Neoplasms - genetics
Endometrial Neoplasms - pathology
endometrioid adenocarcinoma
Endometrium
Endometrium - pathology
Female
Gene Expression Regulation, Neoplastic - genetics
Growth factors
Humans
Immunohistochemistry
Immunoreactivity
IMP3 immunocytochemical expression
Insulin
Middle Aged
mRNA
Neoplasms - diagnosis
Neoplasms - genetics
Neoplasms - pathology
Papanicolaou Test
RNA-Binding Proteins - chemistry
RNA-Binding Proteins - genetics
RNA-Binding Proteins - immunology
serous carcinoma
surface papillary syncytial change
Tumors
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Summary:Introduction This study evaluated the immunocytochemical (ICC) expression of IMP3 in direct endometrial brushings processed as liquid‐based cytology (LBC) samples of endometrioid adenocarcinoma (EAC), serous carcinoma (ESC) and surface papillary syncytial change (SPSC) with endometrial glandular and stromal breakdown (EGBD) to exploit its possible differential diagnostic aid. Methods In total, 333 samples of LBC samples were obtained from selected outpatients in parallel with Pipelle endometrial sampling. They consisted of 97 EAC (83 grade 1: EAC‐1, 14 EAC‐3), 35 ESC and 201 benign endometrial samples (51 proliferative, 42 secretory, 38 atrophic, 70 SPSC with EGBD). ICC expression of insulin‐like growth factor‐II mRNA‐binding protein 3 (IMP3) was manually performed on Papanicolaou‐stained LBC samples. Results The ESC samples showed positive staining cells in 100%, EAC‐3 in 28.5%, and EAC‐1 in 2.4% cases. All the benign endometrium samples were negative. Only ESC cases showed strong immunoreactivity (≥3+) in more than 50% of tumour cells with an average frequency of 80%. Conclusions IMP3 is a helpful immunomarker to distinguish ESC from EAC and SPSC in endometrial cytology. The distinction of high grade endometrial malignancies from functional endometrial atypias and benign endometrial alterations is crucial for appropriate clinical management. In the setting of cytological screening of endometrial carcinomas, IMP3 is a helpful immunomarker to aid separation of serous carcinoma from endometrioid carcinoma and surface papillary syncytial change.
ISSN:0956-5507
1365-2303
DOI:10.1111/cyt.12677