Transcriptional activation of genes involved in oxidative stress in Salmo salar challenged with Piscirickettsia salmonis

Piscirickettsiosis caused by Piscirickettsia salmonis constitutes one of the main problems in farmed salmonid and marine fishes. The objective of this study was to evaluate the modulation of genes involved in the oxidative stress in the liver and muscle of Salmo salar challenge with low dosage of P....

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Published in:Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology 2019-03, Vol.229, p.18-25
Main Authors: Pedro, A.V.F., Martínez, D., Pontigo, J.P., Vargas-Lagos, C., Hawes, C., Wadsworth, S., Morera, F.J., Vargas-Chacoff, L., Yáñez, A.J.
Format: Article
Language:English
Subjects:
Atlantic salmon
Gene expression
Oxidative stress
P. salmonis
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Summary:Piscirickettsiosis caused by Piscirickettsia salmonis constitutes one of the main problems in farmed salmonid and marine fishes. The objective of this study was to evaluate the modulation of genes involved in the oxidative stress in the liver and muscle of Salmo salar challenge with low dosage of P. salmonis. The treatment (in duplicate) were as follows: Control injection (culture medium) and P. salmonis injection (1 × 102 PFU/mL) with sampling (liver and muscle) at several time-points during the 42-days experimental period (dpi). In liver, the gene expression of superoxide dismutase (SOD) and acetylcholinesterase (AChE) had differences with the control group only at 7 dpi, compared with glutathione-S-transferase (GST) and heat shock protein 70 (HSP70) that presented increases at 7 and 21 dpi. The glutathione peroxidase (GPx) and catalase (CAT) mRNAs were elevated at 13 and 21 dpi, respectively. While glutathione reductase (GR) and cytochrome P450 (P450) did not show variations in their expression during the experimental course. In muscle, the expression of CAT and AChE was higher than in the control condition at 2 and 42 dpi, respectively. While the number of transcripts SOD, GPx, GR, GST, P450 and HSP70 showed increases at 7- and 42-days post injection. The results suggest a transcriptional activation of genes involved in oxidative stress in both liver and muscle, with expression profiles that were tissue-specific and dependent on the time. This is the first study that reveals the transcriptional participation of all these genes associated with oxidative stress in response to the injection of P. salmonis. [Display omitted] •Piscirickettsiosis caused by Piscirickettsia salmonis constitutes one of the main problems in farmed salmonid and marine fishes.•The objective of this study was to evaluate the modulation of genes involved in the oxidative stress in the liver and muscle of Salmo salar challenge with low dose of P. salmonis.•The results suggest a transcriptional activation of genes involved in oxidative stress in both liver and muscle.•The gene expression changes in liver and muscle of infected fish suggested that P. salmonis produced a modulation of the expression of genes involved in the oxidative stress response.•The responses to oxidative stress were specific to each tissue analyzed, suggesting that antioxidant defense in liver is more effective to eliminate free radicals than in muscle.
ISSN:1096-4959
1879-1107
DOI:10.1016/j.cbpb.2018.12.004