Changes in Polyamine Content in Rice Bran due to Fermentation with Aspergillus oryzae Analyzed by LC/ESI-MS/MS Combined with Derivatization

Many studies have demonstrated that the dietary supplementation of polyamines, especially spermidine (SPD), prevents age-related diseases. Rice bran is rich in polyamines and their amounts could be increased by fermentation with Aspergillus oryzae (A. oryzae). In this study, we developed a method fo...

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Bibliographic Details
Published in:Analytical Sciences 2019/04/10, Vol.35(4), pp.427-432
Main Authors: HORIE, Yukiko, GOTO, Ayaka, TSUBUKU, Sumi, ITOH, Mari, IKEGAWA, Shigeo, OGAWA, Shoujiro, HIGASHI, Tatsuya
Format: Article
Language:English
Subjects:
Age
Age related diseases
Analytical Chemistry
Aspergillus oryzae
Cell proliferation
Chemistry
derivatization
Diet
Dietary supplements
Fermentation
fermented food
Functional foods & nutraceuticals
Ionization
Ions
LC/ESI-MS/MS
Liquid chromatography
Mass spectrometry
Mass spectroscopy
Oryza
Polyamine
Polyamines
Putrescine
Rice
Rice bran
Spermidine
Spermine
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Summary:Many studies have demonstrated that the dietary supplementation of polyamines, especially spermidine (SPD), prevents age-related diseases. Rice bran is rich in polyamines and their amounts could be increased by fermentation with Aspergillus oryzae (A. oryzae). In this study, we developed a method for the determination of putrescine (PUT), SPD and spermine (SPM) in rice bran samples by liquid chromatography/electrospray ionization–tandem mass spectrometry (LC/ESI-MS/MS) after derivatization with 4-(N,N-dimethylaminosulfonyl)-7-fluoro-2,1,3-benzoxadiazole (DBD-F). The derivatization improved the LC retention and ESI-MS/MS detectability of the polyamines, and consequently enabled precise and accurate quantification. Using this method, we found that the SPD content increased to 158% due to fermentation with A. oryzae, while the content of PUT and SPM decreased. SPD is known as the polyamine playing a central role in cell proliferation and growth, and therefore has health benefits. The fermented rice bran might be a good material for functional foods aimed at SPD supplementation.
ISSN:0910-6340
1348-2246
DOI:10.2116/analsci.18P483