Antibacterial effects of polymeric PolymP-n Active nanoparticles. An in vitro biofilm study

to study the antibacterial effect of polymeric PolymP-n Active nanoparticles using an in vitro subgingival biofilm model. Hydroxyapatite discs coated with five modalities of nanoparticles (NPs): NPs, NPs doped with zinc, calcium, silver and doxycycline, PBS as control, and Streptococcus oralis, Acti...

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Bibliographic Details
Published in:Dental materials 2019-01, Vol.35 (1), p.156-168
Main Authors: Sánchez, M.C., Toledano-Osorio, M., Bueno, J., Figuero, E., Toledano, M., Medina-Castillo, A.L., Osorio, R., Herrera, D., Sanz, M.
Format: Article
Language:English
Subjects:
Actinomyces
Antibiotics
Antiinfectives and antibacterials
Bacteria
Biofilms
Calcium
Dentistry
Doxycycline
Hydroxyapatite
Microscopy
Nanoparticles
Oral biofilm
Physical characteristics
Polymerase chain reaction
Roughness
Silver
Statistical analysis
Variance analysis
Viability
Zinc
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Summary:to study the antibacterial effect of polymeric PolymP-n Active nanoparticles using an in vitro subgingival biofilm model. Hydroxyapatite discs coated with five modalities of nanoparticles (NPs): NPs, NPs doped with zinc, calcium, silver and doxycycline, PBS as control, and Streptococcus oralis, Actinomyces naeslundii, Veillonella parvula, Fusobacterium nucleatum, Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans were studied in a static in vitro biofilm model (12, 24, 48, and 72h). Nano-roughness of the different disc surfaces (SRa, in nm) and morphological characteristic of the biofilms (thickness (μm) and bacterial viability) were studied by different microscopy modalities. Quantitative Polymerase Chain Reaction was used to assess the effect of the nanoparticles on the bacterial load (colony forming unit per milliliter) (CFUmL−1). Analysis of variance and post-hoc testing with T3 Dunnett́s, and Student Newman Keuls correction was used. Results were considered statistically significant at p
ISSN:0109-5641
1879-0097
DOI:10.1016/j.dental.2018.11.015