Evaluation of a tangential flow microfiltration concentration method for the detection of free-living amoebae in water

The recovery efficiency of tangential flow microfiltration for protozoa has been evaluated using water artificially seeded with a free-living amoeba belonging to a non-pathogenic species of Naegleria: N. lovaniensis. After treatment, the recovery of trophozoites from the concentrate was 24±9.5%. Thi...

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Bibliographic Details
Published in:Water research (Oxford) 2000-10, Vol.34 (14), p.3630-3634
Main Authors: Rouby, Y, Garin, D, Pélandakis, M, Mérieux, C, Pernin, P
Format: Article
Language:English
Subjects:
Applied sciences
Drinking water and swimming-pool water. Desalination
Exact sciences and technology
free-living amoebae
Microfiltration
Naegleria
Naegleria lovaniensis
Pollution
Protozoa
tangential microfiltration concentration
Water analysis
Water treatment and pollution
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Summary:The recovery efficiency of tangential flow microfiltration for protozoa has been evaluated using water artificially seeded with a free-living amoeba belonging to a non-pathogenic species of Naegleria: N. lovaniensis. After treatment, the recovery of trophozoites from the concentrate was 24±9.5%. This result was improved further by an elution step which increased final recovery to 40.7±15.2%. This value was significantly better than those we obtained previously for Naegleria trophozoites by standard membrane filtration. Therefore, this method could be applied successfully to the concentration of free-living amoebae in environmental waters.
ISSN:0043-1354
1879-2448
DOI:10.1016/S0043-1354(00)00110-X