Phenol degradation by Ralstonia eutropha: colorimetric determination of 2-hydroxymuconate semialdehyde accumulation to control feed strategy in fed-batch fermentations

Phenol biodegradation by Ralstonia eutropha was modeled in different culture modes to assess phenol feeding in biotechnological depollution processes. The substrate-inhibited growth of R. eutropha was described by the Haldane equation with a K(s) of 2 mg/L, a K(i) of 350 mg/L and a mu(max) of 0.41 h...

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Bibliographic Details
Published in:Biotechnology and bioengineering 1999-11, Vol.65 (4), p.407-415
Main Authors: Leonard, D, Ben Youssef, C, Destruhaut, C, Lindley, N.D, Queinnec, I
Format: Article
Language:English
Subjects:
2-hydroxymuconate semialdehyde
Alcaligenes - metabolism
Bacteria
Batch cell culture
biodegradation
Biodegradation of pollutants
Biodegradation, Environmental
Biological and medical sciences
Bioreactors
Biotechnology
Biotechnology - methods
Calorimetry - methods
Colorimetric analysis
Computer simulation
Environment and pollution
Fatty Acids, Unsaturated - analysis
Fatty Acids, Unsaturated - metabolism
Fermentation
Fundamental and applied biological sciences. Psychology
Haldane equation
Industrial applications and implications. Economical aspects
Kalman filter
Kalman filtering
Mathematical models
meta pathway
Models, Biological
Phenol - metabolism
phenol inhibition
Phenols
Ralstonia eutropha
Toxicity
Two term control systems
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Summary:Phenol biodegradation by Ralstonia eutropha was modeled in different culture modes to assess phenol feeding in biotechnological depollution processes. The substrate-inhibited growth of R. eutropha was described by the Haldane equation with a K(s) of 2 mg/L, a K(i) of 350 mg/L and a mu(max) of 0.41 h(-1). Furthermore, growth in several culture modes was characterized by the appearance of a yellow color, due to production of a metabolic intermediate of the phenol catabolic pathway, 2-hydroxymuconic semialdehyde (2-hms) which was directly correlated to the growth rate and/or the phenol-degradation rate, because these two parameters are coupled (as seen by the constant growth yield of 0.68 g biomass/g phenol whatever the phenol concentration). This correlation between color appearance and metabolic activity was used to develop a control procedure for optimal phenol degradation. A mass-balance equation modeling approach combined with a filtering step using an extended Kalman filter enabled state variables of the biological system to be simulated. A PI controller, using the estimation of the phenol concentration provided by the modeling step, was then built to maintain the phenol concentration at a constant set-point of 0.1 g/L which corresponded to a constant specific growth rate of 0.3 h(-1), close to the maximal specific growth value of the strain. This monitoring strategy, validated for two fed-batch cultures, could lead, in self-cycling fermentation systems, to a productivity of more than 19 kg of phenol consumed/m(3)/d which is the highest value reported to date in the literature. This system of monitoring metabolic activity also protected the bacterial culture against toxicity problems due to the transient accumulation of phenol.
ISSN:0006-3592
1097-0290
DOI:10.1002/(SICI)1097-0290(19991120)65:4<407::AID-BIT5>3.0.CO;2-#