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Elucidating genes involved in sesquiterpenoid and flavonoid biosynthetic pathways in Saussurea lappa by de novo leaf transcriptome analysis
Saussurea lappa (family Asteraceae) possesses immense pharmacological potential mainly due to the presence of sesquiterpene lactones. In spite of its medicinal importance, S. lappa has been poorly explored at the molecular level. We initiated leaf transcriptome sequencing of S. lappa using the illum...
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Published in: | Genomics (San Diego, Calif.) Calif.), 2019-12, Vol.111 (6), p.1474-1482 |
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Main Authors: | , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
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Summary: | Saussurea lappa (family Asteraceae) possesses immense pharmacological potential mainly due to the presence of sesquiterpene lactones. In spite of its medicinal importance, S. lappa has been poorly explored at the molecular level. We initiated leaf transcriptome sequencing of S. lappa using the illumina highseq 2000 platform and generated 62,039,614 raw reads. Trinity assembler generated 122,434 contigs with an N50 value of 1053 bp. The assembled transcripts were compared against the non-redundant protein database at NCBI. The Blast2GO analysis assigned gene ontology (GO) terms, categorized into molecular functions (3132), biological processes (4477) and cellular components (1.927). Using KEGG, around 476 contigs were assigned to 39 pathways. For secondary metabolic pathways, we identified transcripts encoding genes involved in sesquiterpenoid and flavonoid biosynthesis. Relatively low number of transcripts were also found encoding for genes involved in the alkaloid pathway. Our data will contribute to functional genomics and metabolic engineering studies in this plant.
•De novo transcriptome sequencing of S. lappa generated 62,039,614 raw reads which were assembled into 122,434 transcripts.•The transcripts were compared against NR protein database at NCBI and Gene ontology terms were assigned using Blast2GO.•Transcripts encoding genes involved in metabolic pathways like sesquiterpenoid and flavonoid biosynthesis were identified.•The present study provided insight into the molecular networks and putative genes involved in costunolide biosynthesis. |
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ISSN: | 0888-7543 1089-8646 |
DOI: | 10.1016/j.ygeno.2018.09.022 |