Single versus double experimental bile duct ligation model for inducing bacterial translocation

Double common bile duct ligation plus section in rats is used as a model for bacterial translocation, a phenomenon that has been correlated with the degree of liver damage. This study analyzes whether a simpler variant of the technique is also a valid model to study bacterial translocation. Fifty-si...

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Bibliographic Details
Published in:The American journal of surgery 2019-08, Vol.218 (2), p.380-387
Main Authors: Seguí-Ripoll, J.M., Zapater-Hernández, P., Candela-Gomis, A., Compañ-Catalá, L., Francés-Guarinos, R., Payá-Romá, A., Compañ-Rosique, A., Such-Ronda, J.
Format: Article
Language:English
Subjects:
Abdomen
Animals
Bacteria
Bacterial Translocation
Bile
Bile ducts
Bile Ducts - surgery
Cirrhosis
Common Bile Duct - surgery
Cytokines
Disease Models, Animal
E coli
Hypertension
Incidence
Inflammation
Laboratory animals
Ligation
Liver
Liver cirrhosis
Lymph nodes
Male
Phlebotomy
Postoperative Complications - epidemiology
Rats, Sprague-Dawley
Spleen
Translocation
Tumor necrosis factor-TNF
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Summary:Double common bile duct ligation plus section in rats is used as a model for bacterial translocation, a phenomenon that has been correlated with the degree of liver damage. This study analyzes whether a simpler variant of the technique is also a valid model to study bacterial translocation. Fifty-six male Sprague Dawley rats underwent one of three surgical interventions: a) proximal double ligation and section of the common bile duct; b) proximal simple ligation of the bile duct; and c) sham operation. Bacterial translocation was measured by cultures of mesenteric lymph nodes, blood, spleen and liver. Stool culture and histological analysis of liver damage were also performed. The incidence of bacterial translocation in SBL and DBDL groups was 23,5% and 25% respectively. Mortality was similar between ligation groups (11.2% versus 10%). Liver cirrhosis developed in the group of double ligation and section (100% of the animals at 4 weeks), while portal hypertension appeared starting at week 3. None of the animals submitted to simple ligation developed liver cirrhosis. Simple bile duct ligation is associated with a similar incidence of bacterial translocation as double ligation, but without cirrhosis or portal hypertension. •SBL model does not cause cirrhosis, portal hypertension, increased inflammation or bacterial overgrowth as the DBDL model.•SBL model is a good method to study BT at short term with little or no liver damage.•We consider very important to describe the level at which ligatures are performed in each model used.
ISSN:0002-9610
1879-1883
DOI:10.1016/j.amjsurg.2018.09.034