Use of Mixed Self-Assembled Monolayers in a Study of the Effect of the Microenvironment on Immobilized Glucose Oxidase

Mixed self-assembled monolayers (SAMs) composed of a medium length, reactive, n-organothiol (11-mercaptoundecanoic acid) and a short length, unreactive, hydrophobic n-alkylthiol (7-heptanethiol) chemisorbed onto evaporated gold surfaces, were used to study the effect of the microenvironment on the s...

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Bibliographic Details
Published in:Langmuir 1999-02, Vol.15 (4), p.1198-1207
Main Authors: Guiomar, A. Jorge, Guthrie, James T, Evans, Stephen D
Format: Article
Language:English
Subjects:
Angle measurement
Biological and medical sciences
Biosensors
Biotechnology
Carboxylic acids
Catalyst activity
Chemisorption
Contact angle
Cyclic voltammetry
Fundamental and applied biological sciences. Psychology
Gold
Hydrophobicity
Methods. Procedures. Technologies
Monolayers
Various methods and equipments
X ray photoelectron spectroscopy
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Summary:Mixed self-assembled monolayers (SAMs) composed of a medium length, reactive, n-organothiol (11-mercaptoundecanoic acid) and a short length, unreactive, hydrophobic n-alkylthiol (7-heptanethiol) chemisorbed onto evaporated gold surfaces, were used to study the effect of the microenvironment on the structure and activity of immobilized glucose oxidase (GOX). The mixed SAMs were characterized by X-ray photoelectron spectroscopy, contact angle measurements, and cyclic voltammetry. The derivatization of the SAMs for the covalent attachment and the immobilized enzyme were studied by X-ray photoelectron spectroscopy. Quantitative analysis of the amide I band of the infrared spectra of GOX immobilized onto surfaces at the two attainable extremes of the hydrophilicity range available to us indicated that the percentage of β sheet increased with increasing hydrophilicity of the microenvironment. The specific activity of GOX was higher when the enzyme was immobilized onto the hydrophobic microenvironment.
ISSN:0743-7463
1520-5827
DOI:10.1021/la980974t