Isolation of total bacterial DNA for ecological characterization of bottom sediments of Lake Baikal

It is known that the conventional microbiological methods of investigation reveal not more than 1% of the species of microorganisms actually inhabiting one biotope or another. The most complete characterization of the microbial community is achieved by investigating DNA isolated from the samples dif...

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Bibliographic Details
Published in:Contemporary problems of ecology 2008, Vol.1 (1), p.115-119
Main Authors: Chernitsyna, C. M, Shubenkova, O. V, Zemskaya, T. I, Grachev, M. A, Vereshchagin, A. L, Kostornova, T. Ya
Format: Article
Language:English
Subjects:
Bacteria
Biomedical and Life Sciences
Deoxyribonucleic acid
DNA
Ecology
Freshwater ecology
humic substances
isolation techniques
Lakes
Life Sciences
Limnology
microbial communities
microorganisms
Sediments
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Summary:It is known that the conventional microbiological methods of investigation reveal not more than 1% of the species of microorganisms actually inhabiting one biotope or another. The most complete characterization of the microbial community is achieved by investigating DNA isolated from the samples differing from each other in ecological conditions. The sediments of Lake Baikal are characterized by a high content of humic compounds which hinders molecular biological analyses. Because of this, the procedure of DNA isolation is a step o critical importance for the analysis of bottom sediments.In the present work we compare the methods of isolation of the whole bacterial DNA from the bottom sediments of Lake Baikal. The most efficient method allowing us to obtained DNA preparations for further molecular investigation was chosen.
ISSN:1995-4255
1995-4263
DOI:10.1007/s12259-008-1013-1