Coprinus comatus and Ganoderma lucidum interfere with androgen receptor function in LNCaP prostate cancer cells

In this study, we screened a total of 201 diethyl ether, ethanol, and ethyl acetate fungal Basidiomycetes extracts for anti-androgenic activity. Based on our screened results in combination with the selective inhibition of prostate cancer LNCaP cells, we selected Coprinus comatus and Ganoderma lucid...

Full description

Saved in:
Bibliographic Details
Published in:Molecular biology reports 2008-06, Vol.35 (2), p.107-117
Main Authors: Zaidman, Ben-Zion, Wasser, Solomon P, Nevo, Eviatar, Mahajna, Jamal
Format: Article
Language:English
Subjects:
Androgen receptor
Animal Anatomy
Animal Biochemistry
Basidiomycetes
Biomedical and Life Sciences
Cell Line, Tumor
Cell Survival
Cells
Coprinus - metabolism
Coprinus comatus
Drug Screening Assays, Antitumor
Fungi
G1 Phase
Ganoderma - metabolism
Ganoderma lucidum
Genes, Reporter
Histology
Humans
Intracellular Space - metabolism
Life Sciences
LNCaP
Luciferases - metabolism
Male
Molecular biology
Morphology
Original Paper
Prostate cancer
Prostate-Specific Antigen - metabolism
prostatic neoplasms
Prostatic Neoplasms - metabolism
Prostatic Neoplasms - pathology
Receptors, Androgen - genetics
Receptors, Androgen - metabolism
Receptors, Glucocorticoid - genetics
Reproducibility of Results
Studies
Transcriptional Activation - genetics
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:In this study, we screened a total of 201 diethyl ether, ethanol, and ethyl acetate fungal Basidiomycetes extracts for anti-androgenic activity. Based on our screened results in combination with the selective inhibition of prostate cancer LNCaP cells, we selected Coprinus comatus and Ganoderma lucidum for further evaluation. We demonstrated that ethanol and ethyl acetate extracts from C. comatus and G. lucidum, respectively, selectively inhibit dihydrotestosterone-induced LNCaP cell viability, suppress levels of secreted prostate-specific antigen in a dose-dependent manner, and cause a G1 phase arrest in LNCaP, but not in DU 145 and PC-3 cells. For the first time, to the best of our knowledge, we demonstrated that C. comatus and G. lucidum decreased androgen and glucocorticoide receptors transcriptional activity in breast cancer MDA-kb2 cells in a dose-dependent manner, and suppressed androgen receptor (AR) protein level in LNCaP and MDA-kb2 cells. Our findings suggest that AR and non-AR mediated mechanisms underlie the effects of C. comatus and G. lucidum.
ISSN:0301-4851
1573-4978
DOI:10.1007/s11033-007-9059-5