Cationic cyanine chromophore-assembled upconversion nanoparticles for sensing and imaging H2S in living cells and zebrafish

Elevated hydrogen sulfide (H2S) level is closely associated with various diseases. So the sensing of H2S is noteworthy for divulging its role in diagnosing these diseases. Herein, we proposed poly(acrylic acid)-modified upconversion nanoparticles assembled with cationic near-infrared cyanine chromop...

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Bibliographic Details
Published in:Biosensors & bioelectronics 2019-02, Vol.126, p.96-101
Main Authors: Wang, Fangfang, Zhang, Cuiling, Qu, Xuetong, Cheng, Shasha, Xian, Yuezhong
Format: Article
Language:English
Subjects:
Bioimaging
Chromophore
Hydrogen sulfide (H2S)
Turn-on fluorescent probe
Upconversion nanoparticles
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Summary:Elevated hydrogen sulfide (H2S) level is closely associated with various diseases. So the sensing of H2S is noteworthy for divulging its role in diagnosing these diseases. Herein, we proposed poly(acrylic acid)-modified upconversion nanoparticles assembled with cationic near-infrared cyanine chromophores (Cy7-Cl) as the nanoprobe (Cy7-UCNPs) for monitoring H2S based on thiolation reactions. The presence of H2S resulted into about five-fold enhancement in the luminescence intensity of Cy7-UCNPs and the nanoprobe showed a good linearity (R2 =0.9952) over the range of 1.0 − 90 μM. Furthermore, Cy7-UCNPs were successfully employed in sensing and imaging of exogenous and endogenous H2S in live cells and zebrafish. The system shows great potential in the field of nanobiomedicine because of the many excellent properties including high sensitivity, good selectivity, and low cytotoxicity. •A turn-on nanoprobe was developed using Cy7-Cl assembled with core–shell upconversion nanoparticles.•The nanoprobe with NIR input-to-NIR output feature has been applied for highly sensitive and selective detection of H2S.•The nanoprobe had been successfully used in exogenous and endogenous H2S bioimaging in living cells.•The nanoprobe was capable of distinguishing normal and tumor-bearing zebrafish through in vivo imaging of H2S.
ISSN:0956-5663
1873-4235
DOI:10.1016/j.bios.2018.10.056