p53 protein expression levels as bioindicator of individual exposure to ionizing radiation by flow cytometry

Ionizing radiation (IR) can cause various lesions in DNA, which induce the increase of p53 expression levels in order to repair radiation induced damage. Thus, the correlation between the increase of p53 expression and an irradiation may constitute a fast and powerful method of individual monitoring...

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Bibliographic Details
Published in:Molecular and cellular biochemistry 2008, Vol.308 (1-2), p.127-131
Main Authors: Cavalcanti, Mariana Brayner, de Jesus Amaral, Ademir, de Salazar e Fernandes, Thiago, Melo, Jonathan Azevedo, de Faria Machado, Cintia Gonsalves
Format: Article
Language:English
Subjects:
Biochemistry
Biomedical and Life Sciences
Cardiology
Deoxyribonucleic acid
DNA
Dose-Response Relationship, Radiation
Female
Flow Cytometry - methods
Humans
Life Sciences
Lymphocytes
Lymphocytes - radiation effects
Male
Medical Biochemistry
Oncology
Proteins
Radiation
Radiation, Ionizing
Tumor Suppressor Protein p53 - metabolism
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Summary:Ionizing radiation (IR) can cause various lesions in DNA, which induce the increase of p53 expression levels in order to repair radiation induced damage. Thus, the correlation between the increase of p53 expression and an irradiation may constitute a fast and powerful method of individual monitoring in cases of accidental or suspected exposures to IR. In this context, the aim of this research was to evaluate changes in lymphocyte p53 expression levels, based on flow cytometry, after in vitro irradiation of peripheral blood samples. For the measurement of such expression levels of p53 protein, an investigation was carried out in order to establish a methodology of analysis based on flow cytometry. Hence, relationships among levels of expression of p53 protein with the absorbed dose have been verified. The results presented in this report emphasized flow cytometry as an important tool for the fast evaluation of p53 protein expression levels as bioindicator of individual exposure to acute ionizing radiation.
ISSN:0300-8177
1573-4919
DOI:10.1007/s11010-007-9620-5