Determination of naphthalene by competitive fluorescence immunoassay

A reliable and sensitive competitive fluorescence immunoassay for the quantitative determination of naphthalene (NA) was developed. 2-naphthoxy acetic acid (NAA) was selected as the hapten of naphthalene. Active ester method (AEM) was used to couple the NAA to carrier proteins (bovine serum albumin)...

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Bibliographic Details
Published in:	Environmental monitoring and assessment 2009-07, Vol.154 (1-4), p.233-239
Main Authors:	Zhou, Chun, Wang, Qiong-E, Gao, Shan-Shan, Zhuang, Hui-Sheng
Format:	Article
Language:	eng
Subjects:	2-naphthoxy acetic acid Analytical chemistry Animals Antibody Antigens Antigens - chemistry Antigens - immunology Atmospheric Protection/Air Quality Control/Air Pollution Chemistry Chromatography Competition Determination Drinking water Earth and Environmental Science Ecology Ecotoxicology Environment Environmental Management Environmental monitoring Exact sciences and technology Fluorescence Fluorescence immunoassay Glycolates - chemistry Immunoassay Immunoassay - methods Male Methods Mineral oils Miscellaneous Monitoring/Environmental Analysis naphthalene Naphthalenes - analysis Polyclonal antibodies Proteins Rabbits Reagents Studies Water - chemistry Water Pollutants, Chemical - analysis
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Summary:	A reliable and sensitive competitive fluorescence immunoassay for the quantitative determination of naphthalene (NA) was developed. 2-naphthoxy acetic acid (NAA) was selected as the hapten of naphthalene. Active ester method (AEM) was used to couple the NAA to carrier proteins (bovine serum albumin) to form artificial immune antigen. Male New Zealand white rabbits were immunized with this antigen to obtain polyclonal antibodies, with which, a novel fluorescence immunoassay for detection of NA was described. Under best conditions, NA can be determined in the concentration range of 0.1-100 μg/L with a detection limit of 0.05 μg/L. The cross-reactivities of the anti-NA antibody to seven structurally related compounds were below 15%. Some environmental samples were analyzed with satisfactory results. It shows a good accuracy and suitability to analyze NA in environmental water.
ISSN:	0167-6369 1573-2959