Hepatoprotective effect of bisbenzylisoquinoline alkaloid tiliamosine from Tiliacora racemosa in high-fat diet/diethylnitrosamine-induced non-alcoholic steatohepatitis

[Display omitted] •Tiliamosine, a bisbenzylisoquinoline alkaloid was isolated from Tiliacora racemose.•On HepG2 cells, tiliamosine did not exhibit any toxicity up to 100 μM.•In steatotic HepG2 cells, tiliamosine reduced lipid levels and lipotoxicity.•Treatment reduced plasma lipid and transaminase l...

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Bibliographic Details
Published in:Biomedicine & pharmacotherapy 2018-12, Vol.108, p.963-973
Main Authors: Sylvester Darvin, S., Toppo, Erenius, Esakkimuthu, S., Ajeesh Krishna, T.P., Ceasar, S. Antony, Stalin, A., Balakrishna, K., Muniappan, N., Pazhanivel, N., Mahaprabhu, R., Paulraj, M. Gabriel, Pandikumar, P., Ignacimuthu, S., Al-Dhabi, N.A.
Format: Article
Language:English
Subjects:
Alkaloids - pharmacology
Animals
Benzylisoquinolines - pharmacology
Bisbenzylisoquinoline alkaloids
Cell Line, Tumor
Cell Survival - drug effects
Diet, High-Fat - adverse effects
Diethylnitrosamine
Diethylnitrosamine - pharmacology
Hep G2 Cells
HepG2 cells
High fat diet
Humans
Liver - drug effects
Liver - metabolism
Liver Function Tests - methods
Male
Menispermaceae - chemistry
NAFLD
NASH
Non-alcoholic Fatty Liver Disease - chemically induced
Non-alcoholic Fatty Liver Disease - drug therapy
Non-alcoholic Fatty Liver Disease - metabolism
Protective Agents - pharmacology
Rats
Rats, Wistar
Triglycerides - metabolism
Tumor Necrosis Factor-alpha - metabolism
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Summary:[Display omitted] •Tiliamosine, a bisbenzylisoquinoline alkaloid was isolated from Tiliacora racemose.•On HepG2 cells, tiliamosine did not exhibit any toxicity up to 100 μM.•In steatotic HepG2 cells, tiliamosine reduced lipid levels and lipotoxicity.•Treatment reduced plasma lipid and transaminase levels in HFD-DEN induced animals.•Tiliamosine treated animals showed normal liver echogenicity and histology. Non-alcoholic steatohepatitis (NASH) is one of the aggressive forms of non-alcoholic fatty liver disease (NAFLD) and is a potential risk factor of HCC. This study reports the curative effect of tiliamosine on NASH. Tiliamosine was isolated from Tiliacora racemosa Colebr. (Menispermaceae) and its structure was confirmed by studying the physical and spectroscopic data. The effects of tiliamsoine on lipid accumulation and lipotoxicity were evaluated using palmitate-oleate induced steatosis in HepG2 cells. The in vivo efficacy of tiliamosine was evaluated using HFD fed, DEN induced non-alcoholic steatohepatitis Wistar rats. In HepG2 cells, tiliamosine did not affect the cell viability up to 100 μM concentration and showed GI25 value of 264.28 μM. The treatment with tiliamsoine significantly lowered the ORO concentration by 44.17% and triglyceride accumulation by 69.32% at 50 μM concentration (P 
ISSN:0753-3322
1950-6007
DOI:10.1016/j.biopha.2018.09.116