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Degradation of Bupropion: Implications for Interpretation of Postmortem Case Data
The interpretation of postmortem bupropion is often a challenge to the forensic toxicology community because of the instability of the parent compound. At the North Carolina Office of the Chief Medical Examiner (NC OCME) toxicology laboratory, one of the active metabolites, threobupropion, is used a...
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Published in: | Journal of analytical toxicology 2018-10, Vol.42 (8), p.525-536 |
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description | The interpretation of postmortem bupropion is often a challenge to the forensic toxicology community because of the instability of the parent compound. At the North Carolina Office of the Chief Medical Examiner (NC OCME) toxicology laboratory, one of the active metabolites, threobupropion, is used as a complementary indicator for the extent of exposure to the parent compound. Metabolite data will address postmortem normal concentrations as well as when bupropion was attributed to the cause of death. For 55 natural cases where bupropion was unattributed to the cause of death, the blood and liver mean threobupropion concentrations were 1.8 mg/L and 12.1 mg/kg, respectively, with median concentrations of 1.5 mg/L and 10 mg/kg, respectively. For the 51 suicidal ingestion cases when bupropion was attributed to the cause of death, the blood and liver mean threobupropion concentrations were 15.8 mg/L and 131.5 mg/kg, respectively, with median concentrations of 13.5 mg/L and 110 mg/kg, respectively. The laboratory completed a stability study over the course of 50 days to evaluate how bupropion and threobupropion degrade in postmortem blood, liver and liver homogenate. The samples were subjected to forensically relevant conditions by storing them at room temperature (RT, 20°C), refrigerated (4°C) and frozen (-20°C). While the concentration of bupropion decreased in all specimens, the rate of degradation of the RT samples was the most dramatic. The threobupropion metabolite appeared to be relatively stable. The postmortem case data along with the evaluation of potential degradation products should provide an overall picture to assist the toxicological community with the interpretation of bupropion found in routine casework. |
doi_str_mv | 10.1093/jat/bky058 |
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At the North Carolina Office of the Chief Medical Examiner (NC OCME) toxicology laboratory, one of the active metabolites, threobupropion, is used as a complementary indicator for the extent of exposure to the parent compound. Metabolite data will address postmortem normal concentrations as well as when bupropion was attributed to the cause of death. For 55 natural cases where bupropion was unattributed to the cause of death, the blood and liver mean threobupropion concentrations were 1.8 mg/L and 12.1 mg/kg, respectively, with median concentrations of 1.5 mg/L and 10 mg/kg, respectively. For the 51 suicidal ingestion cases when bupropion was attributed to the cause of death, the blood and liver mean threobupropion concentrations were 15.8 mg/L and 131.5 mg/kg, respectively, with median concentrations of 13.5 mg/L and 110 mg/kg, respectively. The laboratory completed a stability study over the course of 50 days to evaluate how bupropion and threobupropion degrade in postmortem blood, liver and liver homogenate. The samples were subjected to forensically relevant conditions by storing them at room temperature (RT, 20°C), refrigerated (4°C) and frozen (-20°C). While the concentration of bupropion decreased in all specimens, the rate of degradation of the RT samples was the most dramatic. The threobupropion metabolite appeared to be relatively stable. The postmortem case data along with the evaluation of potential degradation products should provide an overall picture to assist the toxicological community with the interpretation of bupropion found in routine casework.</description><identifier>ISSN: 0146-4760</identifier><identifier>EISSN: 1945-2403</identifier><identifier>DOI: 10.1093/jat/bky058</identifier><identifier>PMID: 30371844</identifier><language>eng</language><publisher>England</publisher><subject>Antidepressive Agents, Second-Generation - analysis ; Antidepressive Agents, Second-Generation - blood ; Bupropion - analysis ; Bupropion - blood ; Forensic Toxicology - methods ; Gas Chromatography-Mass Spectrometry ; Humans ; Limit of Detection ; Liquid-Liquid Extraction ; Liver - chemistry ; Liver - pathology ; Postmortem Changes ; Reproducibility of Results</subject><ispartof>Journal of analytical toxicology, 2018-10, Vol.42 (8), p.525-536</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c323t-a22e0fbb4e0288d85d4d19b234ee5d99f1aebd37194a1bae1505168b924f716d3</citedby><cites>FETCH-LOGICAL-c323t-a22e0fbb4e0288d85d4d19b234ee5d99f1aebd37194a1bae1505168b924f716d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,786,790,27957,27958</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30371844$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Bishop-Freeman, Sandra C</creatorcontrib><creatorcontrib>Hensel, Erin M</creatorcontrib><creatorcontrib>Feaster, Marc S</creatorcontrib><creatorcontrib>Winecker, Ruth E</creatorcontrib><title>Degradation of Bupropion: Implications for Interpretation of Postmortem Case Data</title><title>Journal of analytical toxicology</title><addtitle>J Anal Toxicol</addtitle><description>The interpretation of postmortem bupropion is often a challenge to the forensic toxicology community because of the instability of the parent compound. At the North Carolina Office of the Chief Medical Examiner (NC OCME) toxicology laboratory, one of the active metabolites, threobupropion, is used as a complementary indicator for the extent of exposure to the parent compound. Metabolite data will address postmortem normal concentrations as well as when bupropion was attributed to the cause of death. For 55 natural cases where bupropion was unattributed to the cause of death, the blood and liver mean threobupropion concentrations were 1.8 mg/L and 12.1 mg/kg, respectively, with median concentrations of 1.5 mg/L and 10 mg/kg, respectively. For the 51 suicidal ingestion cases when bupropion was attributed to the cause of death, the blood and liver mean threobupropion concentrations were 15.8 mg/L and 131.5 mg/kg, respectively, with median concentrations of 13.5 mg/L and 110 mg/kg, respectively. The laboratory completed a stability study over the course of 50 days to evaluate how bupropion and threobupropion degrade in postmortem blood, liver and liver homogenate. The samples were subjected to forensically relevant conditions by storing them at room temperature (RT, 20°C), refrigerated (4°C) and frozen (-20°C). While the concentration of bupropion decreased in all specimens, the rate of degradation of the RT samples was the most dramatic. The threobupropion metabolite appeared to be relatively stable. The postmortem case data along with the evaluation of potential degradation products should provide an overall picture to assist the toxicological community with the interpretation of bupropion found in routine casework.</description><subject>Antidepressive Agents, Second-Generation - analysis</subject><subject>Antidepressive Agents, Second-Generation - blood</subject><subject>Bupropion - analysis</subject><subject>Bupropion - blood</subject><subject>Forensic Toxicology - methods</subject><subject>Gas Chromatography-Mass Spectrometry</subject><subject>Humans</subject><subject>Limit of Detection</subject><subject>Liquid-Liquid Extraction</subject><subject>Liver - chemistry</subject><subject>Liver - pathology</subject><subject>Postmortem Changes</subject><subject>Reproducibility of Results</subject><issn>0146-4760</issn><issn>1945-2403</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><recordid>eNo9kEtLAzEUhYMotlY3_gDJUoSxeU4n7rT1USiooOshmdzI1JlmTDKL_ntHq11dDvfjcPgQOqfkmhLFp2udpuZzS2RxgMZUCZkxQfghGhMq8kzMcjJCJzGuCaF5kfNjNOKEz2ghxBi9LuAjaKtT7TfYO3zXd8F3Q7jBy7Zr6ur3E7HzAS83CUIXIO3pFx9T60OCFs91BLzQSZ-iI6ebCGd_d4LeH-7f5k_Z6vlxOb9dZRVnPGWaMSDOGAGEFYUtpBWWKsO4AJBWKUc1GDvMVEJTo4FKIof5RjHhZjS3fIIud73D4K8eYirbOlbQNHoDvo8loyxXhErBBvRqh1bBxxjAlV2oWx22JSXlj8JyUFjuFA7wxV9vb1qwe_TfGf8GklpttQ</recordid><startdate>20181001</startdate><enddate>20181001</enddate><creator>Bishop-Freeman, Sandra C</creator><creator>Hensel, Erin M</creator><creator>Feaster, Marc S</creator><creator>Winecker, Ruth E</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20181001</creationdate><title>Degradation of Bupropion: Implications for Interpretation of Postmortem Case Data</title><author>Bishop-Freeman, Sandra C ; Hensel, Erin M ; Feaster, Marc S ; Winecker, Ruth E</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c323t-a22e0fbb4e0288d85d4d19b234ee5d99f1aebd37194a1bae1505168b924f716d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Antidepressive Agents, Second-Generation - analysis</topic><topic>Antidepressive Agents, Second-Generation - blood</topic><topic>Bupropion - analysis</topic><topic>Bupropion - blood</topic><topic>Forensic Toxicology - methods</topic><topic>Gas Chromatography-Mass Spectrometry</topic><topic>Humans</topic><topic>Limit of Detection</topic><topic>Liquid-Liquid Extraction</topic><topic>Liver - chemistry</topic><topic>Liver - pathology</topic><topic>Postmortem Changes</topic><topic>Reproducibility of Results</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bishop-Freeman, Sandra C</creatorcontrib><creatorcontrib>Hensel, Erin M</creatorcontrib><creatorcontrib>Feaster, Marc S</creatorcontrib><creatorcontrib>Winecker, Ruth E</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of analytical toxicology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bishop-Freeman, Sandra C</au><au>Hensel, Erin M</au><au>Feaster, Marc S</au><au>Winecker, Ruth E</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Degradation of Bupropion: Implications for Interpretation of Postmortem Case Data</atitle><jtitle>Journal of analytical toxicology</jtitle><addtitle>J Anal Toxicol</addtitle><date>2018-10-01</date><risdate>2018</risdate><volume>42</volume><issue>8</issue><spage>525</spage><epage>536</epage><pages>525-536</pages><issn>0146-4760</issn><eissn>1945-2403</eissn><notes>ObjectType-Article-1</notes><notes>SourceType-Scholarly Journals-1</notes><notes>ObjectType-Feature-2</notes><notes>content type line 23</notes><abstract>The interpretation of postmortem bupropion is often a challenge to the forensic toxicology community because of the instability of the parent compound. At the North Carolina Office of the Chief Medical Examiner (NC OCME) toxicology laboratory, one of the active metabolites, threobupropion, is used as a complementary indicator for the extent of exposure to the parent compound. Metabolite data will address postmortem normal concentrations as well as when bupropion was attributed to the cause of death. For 55 natural cases where bupropion was unattributed to the cause of death, the blood and liver mean threobupropion concentrations were 1.8 mg/L and 12.1 mg/kg, respectively, with median concentrations of 1.5 mg/L and 10 mg/kg, respectively. For the 51 suicidal ingestion cases when bupropion was attributed to the cause of death, the blood and liver mean threobupropion concentrations were 15.8 mg/L and 131.5 mg/kg, respectively, with median concentrations of 13.5 mg/L and 110 mg/kg, respectively. The laboratory completed a stability study over the course of 50 days to evaluate how bupropion and threobupropion degrade in postmortem blood, liver and liver homogenate. The samples were subjected to forensically relevant conditions by storing them at room temperature (RT, 20°C), refrigerated (4°C) and frozen (-20°C). While the concentration of bupropion decreased in all specimens, the rate of degradation of the RT samples was the most dramatic. The threobupropion metabolite appeared to be relatively stable. The postmortem case data along with the evaluation of potential degradation products should provide an overall picture to assist the toxicological community with the interpretation of bupropion found in routine casework.</abstract><cop>England</cop><pmid>30371844</pmid><doi>10.1093/jat/bky058</doi><tpages>12</tpages><oa>free_for_read</oa></addata></record> |
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subjects | Antidepressive Agents, Second-Generation - analysis Antidepressive Agents, Second-Generation - blood Bupropion - analysis Bupropion - blood Forensic Toxicology - methods Gas Chromatography-Mass Spectrometry Humans Limit of Detection Liquid-Liquid Extraction Liver - chemistry Liver - pathology Postmortem Changes Reproducibility of Results |
title | Degradation of Bupropion: Implications for Interpretation of Postmortem Case Data |
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