Degradation of Bupropion: Implications for Interpretation of Postmortem Case Data

The interpretation of postmortem bupropion is often a challenge to the forensic toxicology community because of the instability of the parent compound. At the North Carolina Office of the Chief Medical Examiner (NC OCME) toxicology laboratory, one of the active metabolites, threobupropion, is used a...

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Bibliographic Details
Published in:Journal of analytical toxicology 2018-10, Vol.42 (8), p.525-536
Main Authors: Bishop-Freeman, Sandra C, Hensel, Erin M, Feaster, Marc S, Winecker, Ruth E
Format: Article
Language:English
Subjects:
Antidepressive Agents, Second-Generation - analysis
Antidepressive Agents, Second-Generation - blood
Bupropion - analysis
Bupropion - blood
Forensic Toxicology - methods
Gas Chromatography-Mass Spectrometry
Humans
Limit of Detection
Liquid-Liquid Extraction
Liver - chemistry
Liver - pathology
Postmortem Changes
Reproducibility of Results
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Summary:The interpretation of postmortem bupropion is often a challenge to the forensic toxicology community because of the instability of the parent compound. At the North Carolina Office of the Chief Medical Examiner (NC OCME) toxicology laboratory, one of the active metabolites, threobupropion, is used as a complementary indicator for the extent of exposure to the parent compound. Metabolite data will address postmortem normal concentrations as well as when bupropion was attributed to the cause of death. For 55 natural cases where bupropion was unattributed to the cause of death, the blood and liver mean threobupropion concentrations were 1.8 mg/L and 12.1 mg/kg, respectively, with median concentrations of 1.5 mg/L and 10 mg/kg, respectively. For the 51 suicidal ingestion cases when bupropion was attributed to the cause of death, the blood and liver mean threobupropion concentrations were 15.8 mg/L and 131.5 mg/kg, respectively, with median concentrations of 13.5 mg/L and 110 mg/kg, respectively. The laboratory completed a stability study over the course of 50 days to evaluate how bupropion and threobupropion degrade in postmortem blood, liver and liver homogenate. The samples were subjected to forensically relevant conditions by storing them at room temperature (RT, 20°C), refrigerated (4°C) and frozen (-20°C). While the concentration of bupropion decreased in all specimens, the rate of degradation of the RT samples was the most dramatic. The threobupropion metabolite appeared to be relatively stable. The postmortem case data along with the evaluation of potential degradation products should provide an overall picture to assist the toxicological community with the interpretation of bupropion found in routine casework.
ISSN:0146-4760
1945-2403
DOI:10.1093/jat/bky058