involvement of aquaglyceroporins in transport of boron in barley roots

Boron (B) enters cells as the uncharged boric acid, a small neutral molecule with sufficient lipid solubility to cross cell membranes without the aid of transport proteins. The extent to which the observed uptake rates for B in plants can be explained by this simple physical process was examined by...

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Bibliographic Details
Published in:Plant, cell and environment cell and environment, 2009-10, Vol.32 (10), p.1357-1365
Main Authors: FITZPATRICK, KATE L, REID, ROB J
Format: Article
Language:English
Subjects:
Aquaglyceroporins - genetics
Aquaglyceroporins - metabolism
aquaporin
Biological and medical sciences
Biological Transport
Boron - metabolism
boron transport
Cloning, Molecular
Fundamental and applied biological sciences. Psychology
Gene Expression Regulation, Plant
Genetic Complementation Test
Hordeum - genetics
Hordeum - metabolism
Phylogeny
PIPs
Plant Proteins - genetics
Plant Proteins - metabolism
Plant Roots - genetics
Plant Roots - metabolism
RNA, Plant - genetics
Yeasts - genetics
Yeasts - metabolism
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Summary:Boron (B) enters cells as the uncharged boric acid, a small neutral molecule with sufficient lipid solubility to cross cell membranes without the aid of transport proteins. The extent to which the observed uptake rates for B in plants can be explained by this simple physical process was examined by applying treatments expected to inhibit the membrane transporters most likely to be involved in B transport. These experiments established that at least 50% of B uptake could be facilitated by transporters. The B transport characteristics of two barley aquaglyceroporins, HvPIP1;3 and HvPIP1;4, were investigated using yeast complementation assays. Expression of both genes in yeast resulted in increased B sensitivity. Transport assays in yeast confirmed that HvPIP1;3 and HvPIP1;4 are both capable of transporting B. The physiological role of these HvPIP1 genes in B transport is uncertain since their expression was not responsive to B nutritional status, and they continued to be expressed under toxicity conditions.
ISSN:0140-7791
1365-3040
DOI:10.1111/j.1365-3040.2009.02003.x