Enterococcal Surface Protein Transiently Aggravates Enterococcus faecium–Induced Urinary Tract Infection in Mice

The role that the enterococcal surface protein Esp plays in the capacity of Enterococcus faecium to adhere to uroepithelial cells and the role that it plays in urinary tract infection and peritonitis was investigated in vitro and in vivo, respectively, using Esp-expressing E. faecium (E1162) and its...

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Bibliographic Details
Published in:The Journal of infectious diseases 2009-10, Vol.200 (7), p.1162-1165
Main Authors: Leendertse, Masja, Heikens, Esther, Wijnands, Lucas M., van Luit-Asbroek, Miranda, Teske, Gwendoline J. D., Roelofs, Joris J. T. H., Bonten, Marc J. M., van der Poll, Tom, Willems, Rob J. L.
Format: Article
Language:English
Subjects:
Animals
BACTERIA
Bacterial Adhesion
Bacterial Proteins - genetics
Bacterial Proteins - metabolism
Bacterial Proteins - toxicity
Enterococcus
Enterococcus faecium
Enterococcus faecium - genetics
Enterococcus faecium - metabolism
Epithelial cells
Gene Expression Regulation, Bacterial - physiology
Gram-Positive Bacterial Infections - microbiology
Infections
Kidneys
Membrane proteins
Membrane Proteins - genetics
Membrane Proteins - metabolism
Membrane Proteins - toxicity
Mice
Peritonitis
Protein Binding
Urinary bladder
Urinary tract infections
Urinary Tract Infections - microbiology
Urine
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Summary:The role that the enterococcal surface protein Esp plays in the capacity of Enterococcus faecium to adhere to uroepithelial cells and the role that it plays in urinary tract infection and peritonitis was investigated in vitro and in vivo, respectively, using Esp-expressing E. faecium (E1162) and its isogenic Esp-deficient mutant (E1162Δesp). Esp expression enhanced in vitro binding to bladder and kidney epithelial cells. In mice, higher numbers of E1162 were cultured from kidneys and bladders after the induction of urinary tract infection, compared with E1162Δesp numbers. This was accompanied by a higher frequency of bacteremia, higher cytokine levels in kidney tissue, and renal insufficiency. Esp had no effect on the course of E. faecium peritonitis
ISSN:0022-1899
1537-6613
DOI:10.1086/605609