Rhizosphere microbiome structure alters to enable wilt resistance in tomato

Tomato variety Hawaii 7996 is resistant to the soil-borne pathogen Ralstonia solanacearum, whereas the Moneymaker variety is susceptible to the pathogen. To evaluate whether plant-associated microorganisms have a role in disease resistance, we analyzed the rhizosphere microbiomes of both varieties i...

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Published in:Nature biotechnology 2018-10, Vol.36 (11), p.1100-1109
Main Authors: Kwak, Min-Jung, Kong, Hyun Gi, Choi, Kihyuck, Kwon, Soon-Kyeong, Song, Ju Yeon, Lee, Jidam, Lee, Pyeong An, Choi, Soo Yeon, Seo, Minseok, Lee, Hyoung Ju, Jung, Eun Joo, Park, Hyein, Roy, Nazish, Kim, Heebal, Lee, Myeong Min, Rubin, Edward M, Lee, Seon-Woo, Kim, Jihyun F
Format: Article
Language:English
Subjects:
Cultivars
Disease resistance
Genomes
Microbiomes
Microbiota
Microorganisms
Pathogens
Plant diseases
Plant protection
Probiotics
Rhizosphere
Rhizosphere microorganisms
Soil resistance
Tomatoes
Transplantation
Wilt
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Summary:Tomato variety Hawaii 7996 is resistant to the soil-borne pathogen Ralstonia solanacearum, whereas the Moneymaker variety is susceptible to the pathogen. To evaluate whether plant-associated microorganisms have a role in disease resistance, we analyzed the rhizosphere microbiomes of both varieties in a mesocosm experiment. Microbiome structures differed between the two cultivars. Transplantation of rhizosphere microbiota from resistant plants suppressed disease symptoms in susceptible plants. Comparative analyses of rhizosphere metagenomes from resistant and susceptible plants enabled the identification and assembly of a flavobacterial genome that was far more abundant in the resistant plant rhizosphere microbiome than in that of the susceptible plant. We cultivated this flavobacterium, named TRM1, and found that it could suppress R. solanacearum-disease development in a susceptible plant in pot experiments. Our findings reveal a role for native microbiota in protecting plants from microbial pathogens, and our approach charts a path toward the development of probiotics to ameliorate plant diseases.
ISSN:1087-0156
1546-1696
DOI:10.1038/nbt.4232