Antioxidant and developmental capacity of retinol on the in vitro culture of rabbit embryos

Oxidative stress is a major cause of defective embryo development during in vitro culture. Retinoids are recognized as non-enzymatic antioxidants and may have an important role in the regulation of cell differentiation and vertebrate development. However, there are not enough reports discussing the...

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Bibliographic Details
Published in:Zygote (Cambridge) 2018-08, Vol.26 (4), p.326-332
Main Authors: Elomda, Ahmed M., Saad, Mohamed F., Saeed, Ayman M., Elsayed, Ashraf, Abass, Ahmed O., Safaa, Hosam M., Mehaisen, Gamal M. K.
Format: Article
Language:English
Subjects:
Animals
Antibiotics
Antioxidants
Antioxidants - pharmacology
Biomarkers
Blastocyst - cytology
Blastocyst - drug effects
Cell culture
Cell differentiation
Conflicts of interest
Connexin 43
Differentiation (biology)
Embryo Culture Techniques - methods
Embryo, Mammalian - cytology
Embryo, Mammalian - drug effects
Embryonic Development - drug effects
Embryonic Development - physiology
Embryos
Enzymes
Female
Fertilization in Vitro
Galvanized steel
Gap junctions
Gene expression
Genes
Glutathione
Glutathione peroxidase
Hatchability
Homeobox
Lipid peroxidation
Livestock
Malondialdehyde
Metabolites
Oct-4 protein
Oxidative stress
Penicillin
Peroxidase
Proteins
Rabbits
Retinoids
Superoxide dismutase
Vitamin A
Vitamin A - pharmacology
Vitamins - pharmacology
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Summary:Oxidative stress is a major cause of defective embryo development during in vitro culture. Retinoids are recognized as non-enzymatic antioxidants and may have an important role in the regulation of cell differentiation and vertebrate development. However, there are not enough reports discussing the antioxidant and developmental capacity of retinoids, including retinol (RT), on the in vitro development of embryos recovered from livestock animals, particularly in rabbit species. Therefore, morula embryos obtained from nulliparous Red Baladi rabbit does were cultured for 48 h in TCM199 medium in the absence of RT (control group) or in the presence of RT at concentrations of 10, 100 and 1000 nM. The developmental capacity to the hatched blastocyst stage, the antioxidant biomarker assay and the expression of several selected genes were analyzed in each RT group. The data show that RT significantly (P
ISSN:0967-1994
1469-8730
DOI:10.1017/S0967199418000308