Cryopreservation of collared peccary (Pecari tajacu L., 1758) epididymal sperm using extenders based on Tris and powdered coconut water (ACP®-116c)

The aim of this study was to establish a functional freezing–thawing protocol for epididymal sperm of collared peccaries (Pecari tajacu L., 1758) by comparing different extenders. The epididymal sperm from 12 sexually mature males was recovered by retrograde flushing using Tris-based or coconut wate...

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Bibliographic Details
Published in:Zygote (Cambridge) 2018-08, Vol.26 (4), p.301-307
Main Authors: Bezerra, José A. B., Silva, Andréia M., Sousa, Patrícia C, Campos, Lívia B., Praxedes, Érica C. G., Bezerra, Luana G. P., Castelo, Thibério S., Souza, Ana L. P., Silva, Alexandre R.
Format: Article
Language:English
Subjects:
Animals
Artiodactyla
Cocos - chemistry
Cryopreservation
Cryopreservation - methods
Cryopreservation - veterinary
Cryoprotective Agents - pharmacology
Epididymis - drug effects
Epididymis - physiology
Freezing
Germplasm
Glycerol
Integrity
Laboratories
Male
Males
Melting
Microscopy
Morphology
Pecari tajacu
Plant Extracts - pharmacology
Semen
Semen Analysis
Sperm
Spermatozoa - drug effects
Spermatozoa - physiology
Tayassu
Temperature
Thawing
Thermal resistance
Tromethamine - pharmacology
Viability
Yolk
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Summary:The aim of this study was to establish a functional freezing–thawing protocol for epididymal sperm of collared peccaries (Pecari tajacu L., 1758) by comparing different extenders. The epididymal sperm from 12 sexually mature males was recovered by retrograde flushing using Tris-based or coconut water-based (ACP®-116c) extenders. After initial evaluation, samples were diluted and frozen with the same extenders to which 20% egg yolk and 6% glycerol were added. After 2 weeks, thawing was performed at 37°C/60 s and sperm motility, vigour, morphology, functional membrane integrity, sperm viability, sperm plasma membrane integrity, and a computer-assisted semen analysis (CASA) were assessed. In addition, to evaluate the survival of frozen–thawed sperm, a thermal resistance test (TRT) was executed. Samples preserved using Tris were in better condition compared with those preserved using ACP®, showing higher values for most assessments performed, including CASA and the TRT (P
ISSN:0967-1994
1469-8730
DOI:10.1017/S0967199418000230