Separation of triacylglycerols and free fatty acids in microalgal lipids by solid-phase extraction for separate fatty acid profiling analysis by gas chromatography

Microalgal lipids were separated into two fractions, triacylglycerols (TAGs) and free fatty acids (FFAs), by solid-phase extraction employing sodium carbonate as the sorbent and dichloromethane (20% by volume) in n-hexane as the extracting solvent. The TAG fraction was then saponified, followed by a...

Full description

Saved in:
Bibliographic Details
Published in:Journal of Chromatography A 2009-07, Vol.1216 (31), p.5917-5923
Main Authors: Paik, Man-Jeong, Kim, Hoon, Lee, Jinwoo, Brand, Jerry, Kim, Kyoung-Rae
Format: Article
Language:English
Subjects:
Analytical, structural and metabolic biochemistry
Biological and medical sciences
Free fatty acids
Fundamental and applied biological sciences. Psychology
Gas chromatography
Glycerolipids, phospholipids
Lipids
Microalgae
Other biological molecules
Sodium carbonate
Solid-phase extraction
Triacylglycerols
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Microalgal lipids were separated into two fractions, triacylglycerols (TAGs) and free fatty acids (FFAs), by solid-phase extraction employing sodium carbonate as the sorbent and dichloromethane (20% by volume) in n-hexane as the extracting solvent. The TAG fraction was then saponified, followed by acidification, extraction and tert-butyldimethylsilyl esterification. The FFA fraction was directly acidified, extracted and derivatized. From the lipid extracts of eight microalgal species examined, a total of 13 fatty acids were detected in the TAG fractions and nine were found in the FFA fractions, with at much higher total TAG content in all microalgae. Oleic acid was the most prominent fatty acid in three species, α-linolenic acid was more abundant in two others, and palmitic acid was present in highest concentration in the remaining three species.
ISSN:0021-9673
DOI:10.1016/j.chroma.2009.06.051