Effects of carbon and nitrogen sources on Pleurotus ostreatus ligninolytic enzyme activity

The effect of various carbon and nitrogen sources on laccase, manganese-dependent peroxidase (MnP), and peroxidase production by two strains of Pleurotus ostreatus was investigated. The maximal laccase yield of P. ostreatus 98 and P. ostreatus 108 varied depending upon the carbon source from 5 to 62...

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Bibliographic Details
Published in:World journal of microbiology & biotechnology 2006-09, Vol.22 (9), p.999-1002
Main Authors: MIKIASHVILI, Nona, WASSER, Solomon P, NEVO, Eviatar, ELISASHVILI, Vladimir
Format: Article
Language:English
Subjects:
Bacteria
Biological and medical sciences
Biotechnology
Carbon
Enzymes
Fundamental and applied biological sciences. Psychology
Nitrogen
Pleurotus ostreatus
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Summary:The effect of various carbon and nitrogen sources on laccase, manganese-dependent peroxidase (MnP), and peroxidase production by two strains of Pleurotus ostreatus was investigated. The maximal laccase yield of P. ostreatus 98 and P. ostreatus 108 varied depending upon the carbon source from 5 to 62 U l^sup -1^ and from 55 to 390 U l^sup -1^, respectively. The highest MnP and peroxidase activities were revealed in medium supplemented by xylan. Laccase, MnP, and peroxidase activities of mushrooms decreased with supplementation of defined medium by inorganic nitrogen sources. Peptone followed by casein hydrolysate appeared to be the best nitrogen sources for laccase accumulation by both fungi. However, their positive effects on enzyme accumulation were due to a higher biomass production. The secretion of MnP and peroxidase by P. ostreatus 108 was stimulated with supplementation of casein hydrolysate to the control medium since the specific MnP and peroxidase activities increased 15-fold and 3.5-fold, respectively.[PUBLICATION ABSTRACT]
ISSN:0959-3993
1573-0972
DOI:10.1007/s11274-006-9132-6