In vitro inhibition of the carbonic anhydrase from saanen goat ( Capra hircus) with pesticides

This study was conducted to determine the in vitro effects of four commonly used pesticides (Nuarimol™, Fenarimol™, Parathion-methyl™ and 2,4-D™) on erythrocyte carbonic anhydrase (CA) activity from Saanen goats (SG). The enzyme was 262.57-fold purified by affinity chromatography and the purity was...

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Bibliographic Details
Published in:Pesticide biochemistry and physiology 2007-07, Vol.88 (3), p.307-311
Main Authors: Sinan, Selma, Gencer, Nahit, Turan, Yusuf, Arslan, Oktay
Format: Article
Language:English
Subjects:
2,4-D
adverse effects
Animal productions
Biological and medical sciences
Capra hircus
carbonate dehydratase
Carbonic anhydrase
enzyme activity
enzyme inhibition
erythrocytes
fenarimol
Fundamental and applied biological sciences. Psychology
goats
in vitro studies
inhibitory concentration 50
nontarget organisms
nuarimol
parathion-methyl
Pesticide and inhibition
Phytopathology. Animal pests. Plant and forest protection
Saanen
Saanen goats
Terrestrial animal productions
Vertebrates
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Summary:This study was conducted to determine the in vitro effects of four commonly used pesticides (Nuarimol™, Fenarimol™, Parathion-methyl™ and 2,4-D™) on erythrocyte carbonic anhydrase (CA) activity from Saanen goats (SG). The enzyme was 262.57-fold purified by affinity chromatography and the purity was confirmed by SDS–PAGE. Inhibitory effect of the pesticides on the purified enzyme was determined using the CO 2-hydratase activity method. IC 50 values of the pesticides that caused inhibition were determined by means of activity percentage diagrams. The concentrations of Nuarimol™, Fenarimol™, and 2,4-D™ that inhibited 50% of the enzymatic activity were 0.352, 0.924 and 2.04 mM, respectively. Conversely, the enzyme activity was increased by parathion-methyl.
ISSN:0048-3575
1095-9939
DOI:10.1016/j.pestbp.2007.01.006