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An antifungal peptide from passion fruit ( Passiflora edulis) seeds with similarities to 2S albumin proteins
An actual worldwide problem consists of an expressive increase of economic losses and health problems caused by fungi. In order to solve this problem, several studies have been concentrating on the screening of novel plant defence peptides with antifungal activities. These peptides are commonly char...
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Published in: | Biochimica et biophysica acta 2006-06, Vol.1764 (6), p.1141-1146 |
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Main Authors: | , , , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
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Summary: | An actual worldwide problem consists of an expressive increase of economic losses and health problems caused by fungi. In order to solve this problem, several studies have been concentrating on the screening of novel plant defence peptides with antifungal activities. These peptides are commonly characterized by having low molecular masses and cationic charges. This present work reports on the purification and characterization of a novel plant peptide of 5.0 kDa,
Pe-AFP1, purified from the seeds of passion fruit (
Passiflora edulis). Purification was achieved using a Red-Sepharose Cl-6B affinity column followed by reversed-phase chromatography on Vydac C18-TP column. In vitro assays indicated that
Pe-AFP1 was able of inhibiting the development of the filamentous fungi
Trichoderma harzianum,
Fusarium oxysporum, and
Aspergillus fumigatus with IC
50 values of 32, 34, and 40 μg ml
−
1
, respectively, but not of
Rhyzoctonia solani,
Paracoccidioides brasiliensis and
Candida albicans. This protein was also subjected to automated N-terminal amino acid sequence, showing high degree of similarities to storage 2S albumins, adding a new member to this protein-defence family. The discovery of
Pe-AFP1 could contribute, in a near future, to the development of biotechnological products as antifungal drugs and transgenic plants with enhanced resistance to pathogenic fungi. |
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ISSN: | 1570-9639 0006-3002 1878-1454 1878-2434 |
DOI: | 10.1016/j.bbapap.2006.04.010 |