Proteomic analysis and microtubule dynamicity of human sperm in electromagnetic cryopreservation

The proteomic changes, microtubule dynamicity, and quality parameters of human sperm were investigated during cryopreservation in an extremely low electromagnetic field (ELEF) condition. Semen samples were obtained from 210 healthy individuals with normospermia and then were divided into three exper...

Full description

Saved in:
Bibliographic Details
Published in:Journal of cellular biochemistry 2018-11, Vol.119 (11), p.9483-9497
Main Authors: Gholami, Dariush, Ghaffari, Seyed Mahmood, Shahverdi, Abdolhossein, Sharafi, Mohsen, Riazi, Gholamhossein, Fathi, Rouhollah, Esmaeili, Vahid, Hezavehei, Maryam
Format: Article
Language:English
Subjects:
Cryopreservation
Electromagnetic fields
Electron microscopy
extremely low electromagnetic field
microtubule
Microtubules
Motility
Oxygen
Parameters
Protein structure
Proteins
Proteomics
Reactive oxygen species
Semen
Shotguns
Sperm
Spermatozoa
Tertiary structure
Transmission electron microscopy
Tubulin
Viability
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The proteomic changes, microtubule dynamicity, and quality parameters of human sperm were investigated during cryopreservation in an extremely low electromagnetic field (ELEF) condition. Semen samples were obtained from 210 healthy individuals with normospermia and then were divided into three experimental groups: fresh control, frozen control, and frozen ELEF group. Shotgun proteomics was performed to assess the identification of microtubule proteins of the sperm in experimental groups. Microtubule dynamicity, secondary, and tertiary structure modifications of tubulins, characteristics of transmission electron microscopy of sperm as well as sperm quality parameters were evaluated. The expression ratios of α‐ and β‐tubulins were significantly increased after cryopreservation compared with fresh control while this ratio was not significantly different in frozen ELEF group. The expression ratio of tubulin polymerization‐promoting protein was significantly decreased after cryopreservation compared with fresh control. The length, width, and the activity of microtubule, secondary, and tertiary structures of tubulins, motility, and the viability of the sperm were decreased in frozen control as compared with fresh control. The microtubule activity, secondary, and tertiary structures of sperm tubulin in frozen ELEF group were higher than frozen control. Transmission electron microscopy of microtubules showed that the size of the width and length of the microtubules in frozen ELEF group were greater than frozen control. Motility, viability, and reactive oxygen species levels were improved in frozen ELEF group when compared with frozen control. While the microtubule dynamicity of the sperm was affected by the cryopreservation, this trait was improved during the electromagnetic cryopreservation resulted in better motility and viability. Since the increased viability of sperm cells during the freezing‐thawing process is essential, in this study has attempted to change the network structure of water molecules using the extremely low repetition rate electromagnetic field (ELEF), and investigate the effect of exposed water to ELEF on microtubule dynamicity of sperm and subsequently on motility and viability.
ISSN:0730-2312
1097-4644
DOI:10.1002/jcb.27265