Genetic polymorphism of plastid DNA in Tunisian date-palm germplasm (Phoenix dactylifera L.) detected with PCR-RFLP

Fifteen plastid fragments were amplified from a set of Tunisian date-palm accessions by PCR with consensus primers and analysed by RFLP. Polymorphic DNA bands were obtained as reliable molecular markers to estimate genetic distances among the accessions and to examine their phylogenetic relationship...

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Bibliographic Details
Published in:Genetic resources and crop evolution 2004-08, Vol.51 (5), p.479-487
Main Authors: Hela, S, Salwa, Z, Ali, O.M.S, Abdelmajid, R, Mohamed, M, Mokhtar, T
Format: Article
Language:English
Subjects:
Cultivars
Deoxyribonucleic acid
DNA
Gene polymorphism
Genetic distance
Germplasm
Haplotypes
Phoenix dactylifera
Phylogeny
Polymerase chain reaction
Polymorphism
Primers
Restriction fragment length polymorphism
Typology
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Summary:Fifteen plastid fragments were amplified from a set of Tunisian date-palm accessions by PCR with consensus primers and analysed by RFLP. Polymorphic DNA bands were obtained as reliable molecular markers to estimate genetic distances among the accessions and to examine their phylogenetic relationships. The ctDNA PCR-RFLP method permitted the identification of two haplotypes that differ in the presence or absence of the HinfI restriction site. Phenetic groups composed of cultivars clustered together but does not constitute monophyletic groups. This typology agrees with the haplotypes'' organization and provides a common genetic background within the implied varieties.
ISSN:0925-9864
1573-5109
DOI:10.1023/B:GRES.0000024156.29139.23