Inhibitory effect of probiotic Lactobacillus supernatants from the oral cavity on Streptococcus mutans biofilms

Probiotics can release bioactive substances that can inhibit the growth and biofilm formation of pathogenic microorganisms such as Streptococcus mutans. In this context, we evaluated whether the supernatants of Lactobacillus strains isolated from caries-free subjects can inhibit S. mutans, one of th...

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Bibliographic Details
Published in:Microbial pathogenesis 2018-10, Vol.123, p.361-367
Main Authors: Rossoni, Rodnei Dennis, Velloso, Marisol dos Santos, de Barros, Patrícia Pimentel, de Alvarenga, Janaina Araújo, Santos, Jéssica Diane dos, Santos Prado, Ana Carolina Chipoletti dos, Ribeiro, Felipe de Camargo, Anbinder, Ana Lia, Junqueira, Juliana Campos
Format: Article
Language:English
Subjects:
Anti-Bacterial Agents - metabolism
Anti-Bacterial Agents - pharmacology
Biofilm
Biofilms - drug effects
Biofilms - growth & development
Biomass
Dental Caries - microbiology
Durapatite
Humans
Hydrogen-Ion Concentration
Lactobacillus
Lactobacillus - classification
Lactobacillus - isolation & purification
Lactobacillus - metabolism
Microscopy, Electron, Scanning
Mouth - microbiology
Oral pathogens
Probiotics - metabolism
Probiotics - pharmacology
Streptococcus mutans
Streptococcus mutans - drug effects
Streptococcus mutans - growth & development
Supernatant
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Summary:Probiotics can release bioactive substances that can inhibit the growth and biofilm formation of pathogenic microorganisms such as Streptococcus mutans. In this context, we evaluated whether the supernatants of Lactobacillus strains isolated from caries-free subjects can inhibit S. mutans, one of the most important bacteria for dental caries. First, the supernatants of 22 Lactobacillus strains were screened for antibacterial activity against S. mutans in planktonic cultures. All 22 Lactobacillus strains studied (100%) showed antibacterial activity. Thereafter, the Lactobacillus strains with the greatest reductions in the planktonic S. mutans cultures were tested on biofilms. The L. fermentum 20.4, L. paracasei 11.6, L. paracasei 20.3 and L. paracasei 25.4 strains could significantly reduce the number of S. mutans cells in biofilms formed in hydroxyapatite (p  0.05). In addition, the supernatants of these probiotic strains could also reduce the total biomass of S. mutans biofilms (p 
ISSN:0882-4010
1096-1208
DOI:10.1016/j.micpath.2018.07.032