Molecular characterization and drug susceptibility profile of Mycobacterium tuberculosis isolates from Northeast Bangladesh

Tuberculosis (TB) remains a major public health problem worldwide including in Bangladesh. Molecular epidemiological tools provide genotyping profiles of Mycobacterium tuberculosis (M. tuberculosis) strains that can give insight into the transmission of TB in a specific region. The objective of the...

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Published in:Infection, genetics and evolution genetics and evolution, 2018-11, Vol.65, p.136-143
Main Authors: Uddin, Mohammad Khaja Mafij, Ahmed, Moshtaq, Islam, Mohammad Riazul, Rahman, Arfatur, Khatun, Razia, Hossain, Mohammad Anwar, Maug, Aung Kya Jai, Banu, Sayera
Format: Article
Language:English
Subjects:
Deletion analysis
Drug resistance
Epidemiology
MIRU-VNTR
Mycobacterium tuberculosis
Spoligotyping
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Summary:Tuberculosis (TB) remains a major public health problem worldwide including in Bangladesh. Molecular epidemiological tools provide genotyping profiles of Mycobacterium tuberculosis (M. tuberculosis) strains that can give insight into the transmission of TB in a specific region. The objective of the study was to identify the genetic diversity and drug susceptibility profile of M. tuberculosis strains circulating in the northeast Bangladesh. A total of 244 smear-positive sputum specimens were collected from two referral hospitals in Mymensingh and Netrakona districts. The isolated strains were genotyped by deletion analysis, spoligotyping, and MIRU-VNTR typing. We also analyzed the distributions of drug susceptibility pattern and demographic data among different genotypes. All isolates were identified as M. tuberculosis and among them 167 strains (68.44%) were ‘ancestral’ and the remaining 77 (31.56%) were ‘modern’ type. Spoligotyping analysis yielded 119 distinct patterns, among them, 86 isolates had unique patterns and the remaining 158 were grouped into 33 distinct clusters containing 2 to 18 isolates. The predominant spoligotypes belong to the EAI lineage strains, comprising 66 (27.04%) isolates followed by Beijing (7.38%), T1 (6.15%), CAS1-Delhi (5.33), LAM9 (3.28%), MANU-2 and X2. MIRU-VNTR analysis revealed 167 isolates (68%) had unique patterns, whereas 77 (32%) were grouped into 26 clusters and the rate of recent transmission was 20.9%, suggesting that the majority of TB cases in this region are caused by the reactivation of previous TB infections rather than recent transmission. About 136 (55.7%) isolates were sensitive to four anti-TB drugs, 69 (28.3%) were resistant to one or more (except rifampicin and isoniazid combination) drugs and 39 (15.9%) were MDR. In conclusion, our study provides a first insight into molecular characterization and drug resistance profile of M. tuberculosis strains in northeast Bangladesh which will ultimately contribute to the national TB control program. •Investigation of genetic diversity and drug resistance profile among the M. tuberculosis isolates of Northeast Bangladesh.•Drug sensitive ‘Ancestral’ strains are more prevalently circulating in this area than the ‘modern’ strains.•EAI lineages have been identified as predominant followed by Beijing and T family.•Drug resistances TB are common in Beijing genotype.•Reactivation of previous TB infection plays an important role in TB transmission in northeast part of Bang
ISSN:1567-1348
1567-7257
DOI:10.1016/j.meegid.2018.07.027