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Studies on the genotoxic effect of beryllium chloride and the possible protective role of selenium/vitamins A, C and E
The genotoxic potential of beryllium chloride (BeCl 2) was evaluated in vivo in mice using different endpoints. Chromosomal aberrations in bone marrow cells and in spermatocytes as well as sperm abnormalities were determined in the tested mice. The protective role of an orally administered drug cons...
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Published in: | Mutation research. Genetic toxicology and environmental mutagenesis 2008-04, Vol.652 (2), p.103-111 |
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Main Authors: | , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
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Summary: | The genotoxic potential of beryllium chloride (BeCl
2) was evaluated
in vivo in mice using different endpoints. Chromosomal aberrations in bone marrow cells and in spermatocytes as well as sperm abnormalities were determined in the tested mice. The protective role of an orally administered drug consisting of selenium and vitamins A, C and E (selenium–ACE) was also studied.
For analysis of chromosomal aberrations, both single and repeated oral treatments for a period of 3 weeks were performed. The doses used were 93.75, 187.50, 375, and 750
mg BeCl
2/kg
bw, which corresponds to 1/16, 1/8, 1/4, and 1/2 of the experimental LD
50.
BeCl
2 induced a statistically significant increase in the percentage of chromosomal aberrations in both somatic and germ cells, with a dose– and time–response. The percentage of induced chromosomal aberrations was significantly reduced in all BeCl
2-treated groups after oral administration of selenium–ACE.
Beryllium chloride also induced a significant increase in the percentage of abnormal sperm. This percentage reached values of 9.62
±
0.32 and 5.56
±
0.31 in mice treated with the highest test dose of BeCl
2 and with BeCl
2
+
selenium–ACE, respectively, compared with 1.96
±
0.14 for the control.
In conclusion, the results demonstrate the genotoxic effect of beryllium chloride and confirm the protective role of selenium–ACE against the genotoxicity of beryllium chloride. |
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ISSN: | 1383-5718 1879-3592 |
DOI: | 10.1016/j.mrgentox.2007.12.009 |