Molecular Characterization of a Genomic Fragment Containing Pi-kh Gene from the Genomic Library of Indica Rice Line Tetep

Identification of full length genes along with upstream regulatory elements is important to understand its expression. Here, we report preparation of high titre genomic library and identification of a genomic clone containing Pi-kh gene with its complete upstream and downstream sequences from the ri...

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Bibliographic Details
Published in:Journal of phytopathology 2009-05, Vol.157 (5), p.322-324
Main Authors: Madhav, Maganti S, Plaha, Parikshit, Singh, Nagendra K, Sharma, Tilak R
Format: Article
Language:English
Subjects:
Biological and medical sciences
comparative analysis
Fundamental and applied biological sciences. Psychology
fungal diseases of plants
Magnaporthe grisea
NBS-LRR
Oryza sativa
Phytopathology. Animal pests. Plant and forest protection
resistance gene
rice blast
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Summary:Identification of full length genes along with upstream regulatory elements is important to understand its expression. Here, we report preparation of high titre genomic library and identification of a genomic clone containing Pi-kh gene with its complete upstream and downstream sequences from the rice blast resistant line Tetep. Structural analysis of protein revealed that Pi-kh has a central nucleotide binding site domain, leucine-rich repeats domain and a unique zinc-finger domain. Comparative analysis of Pi-kh protein sequence showed 64% and 45% similarity with the protein sequences of rice blast resistance genes Pi-b and Pi-ta, respectively.
ISSN:0931-1785
1439-0434
DOI:10.1111/j.1439-0434.2008.01487.x