p38 siRNA-encapsulated PLGA nanoparticles alleviate neuropathic pain behavior in rats by inhibiting microglia activation

To investigate whether p38 small-interfering RNA-loaded nanoparticles (p38 siRNA NPs) attenuate spinal nerve ligation (SNL)-induced neuropathic pain in rats by suppressing spinal microglia activation via p38 targeting. After synthesizing p38 siRNA NPs with sonication, physical characteristics were m...

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Bibliographic Details
Published in:Nanomedicine (London, England) England), 2018-07, Vol.13 (13), p.1607-1621
Main Authors: Shin, Juhee, Yin, Yuhua, Park, Hyewon, Park, Seungjo, Triantafillu, Ursula L, Kim, Yonghyun, Kim, Sang Ryong, Lee, Sun Yeul, Kim, Do Kyung, Hong, Jinpyo, Kim, Dong Woon
Format: Article
Language:English
Subjects:
Animals
Disease
Gene expression
Humans
Kinases
Ligation
microglia
Microglia - drug effects
Nanoparticles
Nanoparticles - administration & dosage
Nanoparticles - chemistry
Neuralgia - drug therapy
Neuralgia - genetics
Neuralgia - physiopathology
neuropathic pain
p38 MAPK
p38 Mitogen-Activated Protein Kinases - administration & dosage
p38 Mitogen-Activated Protein Kinases - chemistry
p38 Mitogen-Activated Protein Kinases - genetics
Pain
Phosphorylation
PLGA nanoparticles
Polylactic Acid-Polyglycolic Acid Copolymer - administration & dosage
Proteins
Rats
Rats, Sprague-Dawley
RNA, Small Interfering - administration & dosage
RNA, Small Interfering - chemistry
RNA, Small Interfering - genetics
siRNA
Spinal cord
Spinal Nerves - drug effects
Spinal Nerves - injuries
Spinal Nerves - physiopathology
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Summary:To investigate whether p38 small-interfering RNA-loaded nanoparticles (p38 siRNA NPs) attenuate spinal nerve ligation (SNL)-induced neuropathic pain in rats by suppressing spinal microglia activation via p38 targeting. After synthesizing p38 siRNA NPs with sonication, physical characteristics were measured for size and zeta potential. p38 siRNA NPs were then administrated intrathecally into SNL rats if they could reduce pain behavior excellently. p38 siRNA NPs significantly reduced mechanical allodynia as well as microgliosis in the spinal dorsal horns of SNL rats, consistent with a downregulation of p38-related proinflammatory mediators. As p38 in the spinal microglia plays a critical role in neuropathic pain, we expect that p38 siRNA NPs could be a promising tool for the treatment of neuropathic pain.
ISSN:1743-5889
1748-6963
DOI:10.2217/nnm-2018-0054