DNA-fueled target recycling-induced two-leg DNA walker for amplified electrochemical detection of nucleic acid

Taking advantage of the homogeneous and heterogeneous electrochemical biosensors, a simple, sensitive, and selective electrochemical biosensor is constructed by combining entropy-driven amplification (EDA) with DNA walker. This electrochemical biosensor realizes the biorecognition and EDA operation...

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Bibliographic Details
Published in:Talanta (Oxford) 2018-10, Vol.188, p.685-690
Main Authors: Wang, Kun, Feng, Min, He, Meng-Qi, Zhai, Fu-Heng, Dai, Yu, He, Rong-Huan, Yu, Yong-Liang
Format: Article
Language:English
Subjects:
Biosensing Techniques - methods
Biosensor
Cascade amplification
DNA - blood
DNA - metabolism
DNA walker
DNA, Catalytic - chemistry
DNAzyme motor
Electrochemical Techniques - methods
Electrodes
Entropy-driven amplification
Gold - chemistry
Humans
Immobilized Nucleic Acids - blood
Lead - chemistry
Limit of Detection
Methylene Blue - chemistry
Nanostructures - chemistry
Nucleic Acid Amplification Techniques - methods
Nucleic Acid Hybridization
Reproducibility of Results
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Summary:Taking advantage of the homogeneous and heterogeneous electrochemical biosensors, a simple, sensitive, and selective electrochemical biosensor is constructed by combining entropy-driven amplification (EDA) with DNA walker. This electrochemical biosensor realizes the biorecognition and EDA operation in homogeneous solution, which is beneficial to improve the recognition and amplification efficiency. A two-leg DNA walker generated by EDA can walk on the surface of gold electrode for cleaving the immobilized substrate DNA and releasing the electroactive labels, giving rise to a significant decrease of the electrochemical signal. The immobilization of the electroactive labels ensures the reproducibility and reliability of the biosensor. The present cascade amplification assay can be applied to detect target DNA with a detection limit of 0.29 fM, and base mutations can be easily distinguished. Moreover, the proposed electrochemical biosensor shows a satisfactory performance for the detection of target DNA in human serum. Thus, the novel electrochemical biosensor holds promising potential for a future application in disease diagnosis. [Display omitted] •A new biosensor takes advantages of homogeneous and heterogeneous sensing processes.•An autonomous DNA nanomachine is constructed by combining the EDA and DNA walker.•The electrochemical biosensor offers high sensitivity and selectivity toward DNA.
ISSN:0039-9140
1873-3573
DOI:10.1016/j.talanta.2018.06.049