Molecular cloning of superoxide dismutase (Cu/Zn-SOD) from aquatic molluscs

The potential of the first line of the active oxygen-scavenging system, partial cDNA encoding Cu/Zn superoxide dismutase (SOD) was isolated in three aquatic mollusc species: Ruditapes decussatus (marine clam), Dreissena polymorpha (continental water mussel) and Bathymodiolus azoricus (hydrothermal v...

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Bibliographic Details
Published in:	Marine environmental research 2004-08, Vol.58 (2), p.619-623
Main Authors:	Geret, F., Manduzio, H., Company, R., Leboulenger, F., Bebianno, M.J., Danger, J.M.
Format:	Article
Language:	eng
Subjects:	Amino Acid Sequence Animal and plant ecology Animal, plant and microbial ecology Animals Base Sequence Bathymodiolus azoricus Biological and medical sciences Bivalvia - genetics Bivalvia - metabolism cDNA Conserved Sequence Copper - toxicity DNA Primers DNA, Complementary - genetics Dreissena polymorpha Enzyme Induction - drug effects Fundamental and applied biological sciences. Psychology Marine Molecular Sequence Data Mollusca Molluscs Ruditapes decussatus Sea water ecosystems Sequence Alignment Sequence Analysis, DNA Sequence Homology Superoxide dismutase Superoxide Dismutase - biosynthesis Superoxide Dismutase - genetics Synecology Time Factors
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Summary:	The potential of the first line of the active oxygen-scavenging system, partial cDNA encoding Cu/Zn superoxide dismutase (SOD) was isolated in three aquatic mollusc species: Ruditapes decussatus (marine clam), Dreissena polymorpha (continental water mussel) and Bathymodiolus azoricus (hydrothermal vent mussel). These SOD cDNA fragments were amplified by PCR with degenerate oligonucleotide primers derived from the amino acid sequence conserved in the Cu/Zn-SOD from several other organisms. A partial cDNA of CuZn-SOD was obtained for R. decussates (510 bp), D. polymorpha (510 bp) and B. azoricus (195 bp). The deduced amino acid sequence showed high similarity among the three mollusc species (57–63%) and among other species (50–65%). The residues involved in coordinating copper (His-47, 49, 64, 121) and zinc (His-64, 72, 81 and Asp-84) were well conserved among the three Cu/Zn-SOD sequences.
ISSN:	0141-1136 1879-0291