Injectable platelet rich fibrin: cell content, morphological, and protein characterization

Objectives The aim of the present study was to evaluate the blood cell content, morphological aspects, gene expression of type I collagen, and release of growth factors on an injectable platelet rich fibrin (i-PRF). Materials and methods Blood samples were collected from 15 volunteers to prepare i-P...

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Bibliographic Details
Published in:Clinical oral investigations 2019-03, Vol.23 (3), p.1309-1318
Main Authors: Varela, Hugo Almeida, Souza, Júlio C. M., Nascimento, Rubens M., Araújo, Raimundo F., Vasconcelos, Roseane C., Cavalcante, Rômulo S., Guedes, Paulo M., Araújo, Aurigena A.
Format: Article
Language:English
Subjects:
Adult
Biological activity
Biomaterials
Blood clots
Blood coagulation
Blood platelets
Blood Platelets - cytology
Collagen
Collagen (type I)
Collagen Type I - chemistry
Dentistry
Embedding
Enzyme-linked immunosorbent assay
Fibrin
Fibrin - chemistry
Gene expression
Growth factors
Humans
Immunohistochemistry
Interleukin 10
Interleukin-10 - chemistry
Leukocytes
Leukocytes - cytology
Lymphocytes
Male
Medicine
Morphology
Original Article
Osteocalcin
Osteocalcin - chemistry
Periodontics
Peripheral blood
Platelet-derived growth factor
Platelet-Rich Fibrin - chemistry
Platelet-Rich Fibrin - cytology
Platelets
Polymerase chain reaction
RNA-directed DNA polymerase
Scanning electron microscopy
Transforming Growth Factor beta1 - chemistry
Transforming growth factor-b
Vascular endothelial growth factor
Vascular Endothelial Growth Factor A - chemistry
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Summary:Objectives The aim of the present study was to evaluate the blood cell content, morphological aspects, gene expression of type I collagen, and release of growth factors on an injectable platelet rich fibrin (i-PRF). Materials and methods Blood samples were collected from 15 volunteers to prepare i-PRF samples. Peripheral blood was used as a control group. Blood clot and i-PRF samples were cultured for 10 days. The supernatant of the samples was collected for ELISA immunoassay quantification of PDGF and VEGF growth factors over periods of 1, 8, 24, 72, and 240 h. I-PRF and blood clot samples were biologically characterized using histological and immunohistochemistry analysis for IL-10, osteocalcin, and TGF-β. Scanning electron microscopy (SEM) was used to inspect the fibrin network and distribution of blood platelets and leukocytes. Reverse transcriptase polymerase chain reaction (RT-PCR) method was used to evaluate gene expression for type I collagen. Results A higher concentration of platelets and lymphocytes was recorded in i-PRF than in peripheral blood ( p  
ISSN:1432-6981
1436-3771
DOI:10.1007/s00784-018-2555-2